郑州大学学报（医学版）2017，Vol.52Issue(2)：134-137,4.DOI:10.13705/j.issn.1671-6825.2017.02.007
脱氧雪腐镰刀菌烯醇的高灵敏化学发光磁酶免疫法检测
Determination of deoxynivalenol by a sensitive method of magnetic en-zyme chemiluminescence immunoassay
摘要
Abstract
Aim: To propose a magnetic enzyme chemiluminescence immunoassay ( MECLIA ) for rapid and sensitive detection of deoxynivalenol (DON).Methods: Fe3O4@SiO2@Au particles and the conjugation of DON to ovalbumin (OVA-DON) were prepared.Then,the DON-OVA was coated on the surface of Fe3O4@SiO2@Au by using Fe3O4@SiO2@Au as immobile phase.After that, MECLIA based on the luminol-H2O2-HRP-phenols system for detection of DON was established.The accuracy and sensitivity of the established method were evaluated .Results:The standard curve equation was Y=-521951 X+2883979 ( X was the logarithm of the concentration of DON , Y was the illumination value of the standard concentration, R2 =0.9986), the linear range was 0.10~20.00 μg/L, the limit of detection was 31 ng/L, the relative standard deviations were less than 3.5% and 9.6% for intra-and inter-assay, respectively, and the recoveries ranged from 95.0%to 108.0%.The proposed method was satisfactorily applied to determine DON in wheat sample and the recoveries ranged from 92.0%to 106.0%.Conclusion:The developed MECLIA is sensitive and simple with good preci-sion for DON residue analysis in wheat samples.关键词
脱氧雪腐镰刀菌烯醇/金磁复合微粒/化学发光磁酶免疫法Key words
deoxynivalenol/gold magnetic partical/magnetic enzyme chemiluminescence immunoassay分类
医药卫生引用本文复制引用
丁丽华,于斐,张冠军,熊亚敏,刘利娥,吴拥军,玉崧成..脱氧雪腐镰刀菌烯醇的高灵敏化学发光磁酶免疫法检测[J].郑州大学学报（医学版）,2017,52(2):134-137,4.基金项目
国家自然科学基金青年项目 81402721 （）
河南省教育厅自然科学基础研究项目 14A330008 （）
2014郑州大学研究生教育科研专项基金资助项目 （）
