郑州大学学报（医学版）2017，Vol.52Issue(2)：138-142,5.DOI:10.13705/j.issn.1671-6825.2017.02.008
Lgr5、β-catenin在卵巢上皮性癌组织中的表达及对SKOV3细胞增殖迁移的影响
Expressions of Lgr5 andβ-catenin in epithelial ovarian cancer tissue and the effect on cell proliferation and migration of SKOV 3 cells
摘要
Abstract
Aim: To investigate the expressions of G protein coupled receptor Lgr 5 andβ-catenin in ovarian epithelial cancer tissue and the effect on the cell proliferation and migration of SKOV 3 cells.Methods:The expressions of Lgr5 andβ-catenin protein in tissue from 30 cases of ovarian cancer and 30 cases of ovarian benign tumor , as well as 30 normal ovar-ian tissue samples were detected by immunohistochemistry .The SKOV3 cells were transfected with Lgr 5 shRNA, and those transfected with NC shRNA and without transfection were the control .The protein and mRNA expressions of Lgr 5 and β-catenin in SKOV3 cells were detected by Western blot and qRT-PCR,respectively;cell proliferation was detected by CCK-8 assay and the cell migration was detected by scratch assay .Results:The positive rates of Lgr 5 andβ-catenin in epitheli-al ovarian cancer tissue were 63.3%(19/30) and 83.3%(25/30), which were higher when compared with the normal o-varian tissue and ovarian benign tumor tissue (P<0.05).There was positive correlation between the expressions of Lgr 5 andβ-catenin in epithelial ovarian cancer tissue (rp =0.373, P=0.028).Compared with the two control groups, the protein and mRNA expressions of Lgr5 andβ-catenin in SKOV3 cells transfected with Lgr5 shRNA were significantly lower ( P<0.05), and proliferation and migration capacity were significantly lower (P<0.05).Conclusion: Lgr5 may regulate the proliferation and migration of SKOV 3 cells by enhancing the signal pathway of Wnt /β-catenin.关键词
Lgr5/Wnt/β-catenin信号通路/卵巢上皮性癌/细胞增殖/细胞迁移/SKOV3细胞Key words
Lgr5/Wnt/β-catenin signaling pathway/epithelial ovarian cancer/cell proliferation/cell migration/SKOV3 cells分类
医药卫生引用本文复制引用
张展,朱海,宋华,王金铭..Lgr5、β-catenin在卵巢上皮性癌组织中的表达及对SKOV3细胞增殖迁移的影响[J].郑州大学学报（医学版）,2017,52(2):138-142,5.基金项目
河南省科技厅基础与前沿项目 152300410190 （）
