郑州大学学报（医学版）2017，Vol.52Issue(2)：159-163,5.DOI:10.13705/j.issn.1671-6825.2017.02.013
赤芍醇提物对血管内皮细胞一氧化氮合成和分泌的影响
Effect of ethanol extract of Paeonia veitchii Lynch on production and se-cretion of nitric oxide in HUVECs
摘要
Abstract
Aim: To investigate the effect of 95%ethanol extract of Paeonia veitchii Lynch( PVL) on production and secretion of nitric oxide ( NO) in cultured human umbilical vein endothelium cells ( HUVECs ) .Methods: The HUVECs were incubated in different concentration (0.025, 0.050, 0.100 g/L) PVL in vitro, respectively.The content of NO inside and outside HUVECs were measured by DAF-FM DA and Griess reaction .The expression levels of Akt , eNOS, p-Akt Ser473 , p-eNOS Ser1177 and p-eNOS Thr495 in HUVECs cultured with 0.100 g/L PVL for 0,2,5,10,15,30,60,120 min were assessed by Western blot .Results:PVL increased the extracellular and intracellular NO production in a dose-dependent manner in cultured HUVECs(P<0.05).PVL also changed the phosphorylation of Akt Ser 473, eNOS Ser1177 and dephos-phorylation of eNOS Thr495 in HUVECs at different time points(P<0.05).Conclusion:PVL could increase the NO pro-duction in HUVECs , thus play a role of endothelium dependent vasodilation , and the mechanism may be related to the acti-vation of Akt-eNOS-NO signal transduction pathway.关键词
赤芍/蛋白激酶B/内皮型一氧化氮合酶/一氧化氮/人脐静脉内皮细胞Key words
Paeonia veitchii Lynch/protein kinase B/endothelial nitric oxide synthase/nitric oxide/human umbilical venus endothelial cell分类
医药卫生引用本文复制引用
史海霞,杨嘉君,聂绪强,薛永亮,卞卡..赤芍醇提物对血管内皮细胞一氧化氮合成和分泌的影响[J].郑州大学学报（医学版）,2017,52(2):159-163,5.基金项目
上海市科委生物医药重点项目子课题 14401970303 （）
上海交通大学医学院附属第三人民医院科研项目 syz2011-02 （）
上海交通大学附属第六人民医院预研基金 LYZY-0175 （）
