郑州大学学报（医学版）2017，Vol.52Issue(2)：163-167,5.DOI:10.13705/j.issn.1671-6825.2017.02.014
可溶性环氧化物水解酶抑制剂在TGF-β1诱导的人脐静脉内皮细胞内皮-间质转化中的作用
Effect of soluble epoxide hydrolase inhibitor on endothelial -to-mesenchymal transition of human umbilical vein endothelial cells induced by TGF-β1
摘要
Abstract
Aim: To observe the effect of soluble epoxide hydrolase inhibitor t-AUCB on TGF-β1 induced endothelial-to-mesenchymal transition(EndMT) of human umbilical vein endothelial cell(HUVEC).Methods:The HUVEC were pre-treated by t-AUCB(50 μmol/L) for 40 min,then were induced by TGF-β1(10 μg/L) for 24 h,and Western blot was used to detect the phosphorylation levels of Smad 2/3;after 72 h, light microscope was used to observe cell morphology , CCK-8 kit was used to test cell viability , and Real-time PCR was used to determine the mRNA expressions of epithelial marker (CD31) and mesenchymal markers(collagen Ⅰ,collagen Ⅲ,vimentin), as well as the key downstream transcription fac-tors(snail1,twist1,twist2,ZEB1).TGF-β1 group and t-AUCB group were set.Results: The cells in TGF-β1 group changed into a long and narrow shape with intercellular porosis , while the cells in TGF-β1+t-AUCB group and t-AUCB group had normal forms.In TGF-β1 group,CD31 mRNA expression was down-regulated, but those of collagen Ⅰ, collagenⅢ, vimentin,snail1,twist1,twist2,and ZEB1 were up-regulated,and Smad2 and Smad3 protein phosphorylation levels in-creased(P <0.05).Compared with those of TGF-β1 group, the indexes mentioned above in TGF-β1 +t-AUCB group andt-AUCB group showed reversed effect to a certain extent (P <0.05).Conclusion: t-AUCB has an anti-fibrosis effect on theHUVEC by inhibiting the progress of EndMT.关键词
可溶性环氧化物水解酶抑制剂/内皮-间质转化/脐静脉内皮细胞/心肌纤维化Key words
soluble epoxide hydrolase inhibitor/endothelial-to-mesenchymal transition/HUVEC/myocardial fibrosis分类
医药卫生引用本文复制引用
郭亚男,杨海波,赵荫涛,刘源,李凌,周岩强..可溶性环氧化物水解酶抑制剂在TGF-β1诱导的人脐静脉内皮细胞内皮-间质转化中的作用[J].郑州大学学报（医学版）,2017,52(2):163-167,5.基金项目
郑州大学第一附属医院院内青年创新基金项目 （）
