郑州大学学报（医学版）2017，Vol.52Issue(2)：213-216,4.DOI:10.13705/j.issn.1671-6825.2017.02.027
2-甲氧基雌二醇及其合成中间体的大鼠肺细胞膜色谱行为
Establishment of rat lung cell membrane chromatography and chromato-graphic affinity of 2-methoxyestradiol and synthetic derivatives
张青青 1张苗 2尚彩红 1周建华 1郭丽芳 1杜斌3
作者信息
- 1. 郑州大学第二附属医院药剂科 郑州 450014
- 2. 沈阳药科大学药学院药物制剂教研室 沈阳 110016
- 3. 郑州大学药学院药物分析教研室 郑州 450001
- 折叠
摘要
Abstract
Aim: To screen the activity of 2-methoxyestradiol (2-ME) and synthetic derivatives by rat lung cell mem-brane chromatography .Methods:The lung cell membrane stationary phase ( CMSP ) was packed into a stainless steel col-umn(10 mm ×2 mm), and the HPLC conditions were as follows:a mobile phase consisted of 10 mmol/L phosphate buffer (pH 7.4) at a flow rate of 0.2 mL/min, and the column temperature was 37 ℃.Salbutamol was used as positive control in the screening .The capacity factor k′as chromatographic parameters was used to describe the affinity of 2-ME and its synthetic derivatives .Results:2-ME,NO.1 synthetic derivative and NO .2 synthetic derivative had chromatographic reten-tion on lung CMSP column and k′of NO.2 was greater than NO.1 and 2-ME.The k′of 2-ME with rat lung CMSP was higher than those with pure silica gel column ,rat cardiac muscle CMSP and liver CMSP .Conclusion:2-ME has the proper-ty of specific affinity on the membrane of the rat lung cell membrane.关键词
2-甲氧基雌二醇/细胞膜色谱/靶向性Key words
2-methoxyestradiol/cell membrane chromatography/targeting分类
医药卫生引用本文复制引用
张青青,张苗,尚彩红,周建华,郭丽芳,杜斌..2-甲氧基雌二醇及其合成中间体的大鼠肺细胞膜色谱行为[J].郑州大学学报（医学版）,2017,52(2):213-216,4.
