中国实用神经疾病杂志2017，Vol.20Issue(7)：3-6,4.
microRNA-183通过下调Bcl-2诱导人神经母细胞瘤细胞株SK-N-SH细胞凋亡的研究
microRNA-183 induces apoptosis of human neuroblastoma cell line SK-N-SH by down regulating Bcl-2 disease rats
马国祥 1丁茜萍 1邓海华 1杨振汉1
作者信息
- 1. 广东深圳市宝安区福永人民医院内科 深圳 518103
- 折叠
摘要
Abstract
Objective To study the regulation of microRNA-183 on neuroblastoma cells and to provide a new strategy for the treatment of neuroblastoma.Methods microRNA and microRNA-183 were transfected into SK-N-SH cells,and then MTT experiment and Caspase-3 activity assay and flow cytometry were employed to investigate the role of microRNA-183 in growth and apoptosis of human neuroblastoma cell line SK-N-SH.Bcl-2 siRNA was transfected into SK-N-SH cells,and effects of Bcl-2 on hu-man neuroblastoma cell line SK-N-SH cells apoptosis was tested.After transfection of Bcl-2 plasmid,microRNA-183 was trans-fected into human neuroblastoma cell line SK-N-SH cells,and the expression level of Bcl-2 and apoptosis of human neuroblastoma cell line SK-N-SH were analyzed.Results The transfection of microRNA-183 reduced cell growth of the SK-N-SH cells (P=0.0059),and the expression of phosphatidylserine on the membrane (P=0.008)and activation of Caspase-3 were observed (P=0.014),and the expression of Bcl-2 was decreased (P=0.015).Bcl-2 interference of human neuroblastoma cell lines enhances ap-optosis of the human neuroblastoma cell line SK-N-SH induced by microRNA-183 (P=0.0058),and overexpression of Bcl-2 in-hibits apoptosis induced by microRNA-183 (P=0.0073).Conclusion The transfection of microRNA-183 inhibits the growth and proliferation of human neuroblastoma cell line SK-N-SH.MicroRNA-183 induces apoptosis of SK-N-SH by down regulating Bcl-2, which suggests that Bcl-2 may be a potential candidate therapeutic target for neuroblastoma.关键词
细胞凋亡/microRNA-183/Bcl-2/SK-N-SH细胞Key words
Apoptosis/microRNA-183/Bcl-2/SK-N-SH cell分类
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马国祥,丁茜萍,邓海华,杨振汉..microRNA-183通过下调Bcl-2诱导人神经母细胞瘤细胞株SK-N-SH细胞凋亡的研究[J].中国实用神经疾病杂志,2017,20(7):3-6,4.
