湖南中医药大学学报2017，Vol.37Issue(3)：254-258,5.DOI:10.3969/j.issn.1674-070X.2017.03.006
补肾化瘀方对PCOS大鼠子宫内膜整合素αvβ3及LIF表达的影响
Effects of Bushen Huayu Precription on the Expression of Endometrial Integrin αvβ3 and LIF in PCOS Rats
摘要
Abstract
Objective To explore the influence of Bushen Huayu prescription on endometrial integrin-αvβ3 and leukaemia inhibitory factor(LIF) in polycystic ovary syndrome(PCOS) rats. Methods One hundred SD rats were randomly devided into two groups of 25 rats in control group and 75 rats in model group. PCOS rat modules were to built by inject-ing dehydroepiandrostexone (DHEA) solution in the back of the neck subcutaneous of rats. After modules built successfully, the selected rats were randomly divided into model group, diane-35 group and Bushen Huayu prescription treatment group, given by gavage with distilled water, diane-35 and Bushen Huayu prescription separately for 21 d. Then stopped 7 d for a cycle. Drug withdraw after 3 discontinuous cycles, female rats were cohabited with male rats according to 1:1. The rats were executed after pregnancy for 4 d. The expressions of endometrial integrin-αvβ3 and LIF were detecting using immunohistomchemi-cal method in PCOS rats. Results (1) Compared with the control group, the expressions of the integrin-αvβ3 were lower in model group, diane-35 group and Bushen huayu prescription group (P<0.05). Compared with the module group, the expres-sions of the integrin-αvβ3 were higher in Bushen Huayu prescription group (P<0.05) and were lower in diane-35 group (P>0.05). (2) Compared with the control group, the expressions of the LIF were lower in model group, diane-35 group (P<0.05). Com-pared with the module group, the expressions of the LIF were higher in Bushen Huayu prescription group (P<0.05). Conclusion Bushen Huayu prescription can increase the expressions of endometrium integrin-αvβ3 and LIF in PCOS rats, that may be one of the mechanisms of improving the receptivity of endometrium.关键词
多囊卵巢综合征/子宫内膜容受性/整合素/白血病抑制因子/补肾化瘀Key words
polycystic ovary syndrome/endometrial receptivity/integrin/leukemia inhibitory factor/tonifying kidney and removing blood stasis/Bushen Huayu prescription分类
医药卫生引用本文复制引用
罗佩,侯丽莹,邓丽玲,赵娜,全春梅,杨正望,余曦明..补肾化瘀方对PCOS大鼠子宫内膜整合素αvβ3及LIF表达的影响[J].湖南中医药大学学报,2017,37(3):254-258,5.基金项目
湖南省科技厅计划一般项目(2014TT2007). （2014TT2007）
