中医药导报2017，Vol.23Issue(3)：11-14,4.
木犀草素体外对H1N1感染A549作用的研究
Intervention Effect of Luteolin on A549 infected by H1N1 in Vitro
摘要
Abstract
Objective:To observe the role of Luteolin after the infection of influenza virus (H1N1) in human lung cancer cell line (A549 cell line).Methods:The maxinum of non-toxic concentration (TC0) and the median toxic concentration (TC50) were detected by the cytopathogenic effect (CPE) of cells.Furthermore,The median inhibitory concentration (IC50) and treatment indexes (TI) in the best mode were calculated by Probit regression.Results:The median toxic concentration of Luteolin was 39.810 μg·mL-1,and maximum non-toxic concentration was 25 μg·mL-1;A significant inhibitory effect was shown when added luteolin from 12.5 to 25.0 μg·mL-1 after 48-60 h compared with the model without the drug.In addition,the best method against viral infection was adding Luteolin at 2 after cells infected and the best survival rate was (87.00±1.71) ％ with 25.0 μg·mL-1 of Luteolin.Under this condition,IC50 was 11.282 μg·mL-1,and TI was 3.528.Conclusion:Luteolin possesses the effect of anti-H1NI virus in vitro.关键词
木犀草素/体外/A549/H1N1Key words
Luteolin/invirto/A549/H1N1分类
医药卫生引用本文复制引用
邓东沅,顾立刚,刘晓婷,于卓男,吴珺,邱泽计,王玥琦..木犀草素体外对H1N1感染A549作用的研究[J].中医药导报,2017,23(3):11-14,4.基金项目
国家自然科学基金资助项目(No.81173371) （No.81173371）
