四川大学学报（医学版）2017，Vol.48Issue(2)：186-190,5.
细胞自噬在三氧化二砷诱导肝癌细胞凋亡中的作用研究
Effect of Cell Autophagy on the Apoptosis of Hepatocellular Carcinoma Cells Induced by Arsenic Trioxide
摘要
Abstract
Objective To determine the effect of autophagy on the apoptosis of hepatocellular carcinoma cells induced by arsenic trioxide (ATO).Methods Hepatocellular carcinoma HepG2 cells were exposed to ATO.The cell viability was detected by MTT after adjustments for autophagy agonist (Rap) and autophagy inhibitor (3-MA).The autophagosome was observed under electronic microscope.The autophagy related proteins (LC3 and Beclinl) were detected by immunofluorescence.The cell apoptosis was measured by flow cytometry.Results With 5-20 μmol/L of ATO,HepG2 cells exposed to 3-MA had significantly lower viability (P＜0.05) and higher early apoptosis (P＜0.05) than those without exposure to 3-MA.Exposure to 3-MA was also associated with lower expressions of LC3 and Beclin1,with HepG2 cells showing typical apoptotic characteristics.By contrast,with 5-20 μmol/L of ATO,the cells exposed to Rap showed significantly higher viability (P＜ 0.05) and lower early apoptosis (P＜0.05) than those without exposure to Rap.A large number of autophagosome appeared in the cells exposed to Rap.Exposure to Rap was associated with increased expressions of LC3 and Beclin1,but with no statistical significance (P ＞ 0.05).Conclusion Targeted autophagy inhibition can significantly increase the sensitivity of HepG2 to ATO.The underlining mechanism is associated with enhanced apoptosis of hepatocellular carcinoma cells.关键词
肝细胞癌/三氧化二砷/自噬/凋亡Key words
Hepatocellular carcinoma/Arsenic trioxide/Autophagy/Apoptosis引用本文复制引用
李洋,谷仕艳,张遵真..细胞自噬在三氧化二砷诱导肝癌细胞凋亡中的作用研究[J].四川大学学报（医学版）,2017,48(2):186-190,5.基金项目
国家自然科学基金面上项目(No.81372945)和四川省卫计委科研课题(No.150109)资助 （No.81372945）
