四川大学学报（医学版）2017，Vol.48Issue(2)：203-209,7.
p-JAK1/p-STAT3通路在蓝莓益生菌血清干预肝细胞脂肪变性中的作用
The Effect of p-JAK1/p-STAT3 Signaling Way in Intervening NAFLD by Blueberry Probiotic Serum
摘要
Abstract
Objective To explore the mechanism of interlukin-22 (IL-22)-mediated phosphor-Janus kinase-1 (p-JAK1)/phosphor-signal transducer and activator of transcription 3 (p-STAT3) signaling way in the experimcnt of improving non-alcholic fatty liver disease (NAFLD) by blueberry probiotic serum.Methods The rat serums with low-,medium-,and high-dose of 10％ blueberry probiotics,as well as saline were prepared.NAFLD model was built by inducing normal liver cell line L-02 with free fatty acid (FFA).NAFLD model cells were cultured with saline serum (model group),low-,medium-,and high-dose blueberry probiotics serums (low-,medium-,and high-dose serum groups),respectively.Normal liver cell group (normal group) was cultured with saline serum.Oil Red O staining was used to detect the lipid deposition in the cells;the intracellular level of triglyceride (TG) was quantitatively determined;the gene and protein expressions of IL-22,p-JAK1,p-STAT3,sterol-regulatory element binding protein-lc (SREBP-lc) were detected by RT-PCR,Western blot and immunofluorescence methods.Results Twenty-four hours after modeling,a large amount of lipid deposition could be observed in model group.Compared with normal group,model group showed lower gene and protein expression levels of IL-22,p-JAK1 and p-STAT3 (P＜0.01),and higher SREBP-1c and TG levels (P＜0.01).Compared with model group,TG level and the lipid deposition in low-,medium-,and high-dose blueberry probiotics serum groups were gradually reduced.High-dose serum group showed higher gene and protein expression levels of IL-22,p-JAK1,p-STAT3 and lower SREBP-1c compared with the model,low-,and medium-dose serum groups (P＜0.01).No significant differences in gene and protein levels between low-and medium dose serum groups were found (P＞0.05).Conclusion The blueberry probiotics could antagonize the NAFLD via ρ-JAK1/p-STAT3 signaling way.关键词
蓝莓益生菌血清/肝细胞/脂肪变性/白介素-22/磷酸化酪氨酸蛋白激酶-1/磷酸化信号转导激活转录因子-3/信号通路Key words
Blueberry probiotics serum/Hepatocyte/Steatosis/IL-22/p-JAK1/p-STAT3/Signaling way引用本文复制引用
祝娟娟,程明亮,周明玉,赵雪珂..p-JAK1/p-STAT3通路在蓝莓益生菌血清干预肝细胞脂肪变性中的作用[J].四川大学学报（医学版）,2017,48(2):203-209,7.基金项目
贵州省科技厅社会攻关项目(No.黔科合SY[2010] 3017号)、黔东南苗族侗族自治州林业局科研基金项目(No.ZLYJZFCG-2012-7)和贵州省卫计委科技项目(No.gzwjkj2016-1-02)资助 （No.黔科合SY[2010] 3017号）
