四川大学学报（医学版）2017，Vol.48Issue(2)：276-281,6.
18F-NaF PET-CT显像技术辅助下的前列腺癌骨转移动物模型建立
Establishment of Bone Metastasis Model of Prostate Cancer in Mice with Monitoring of 18F-NaF PET-CT
摘要
Abstract
Objective To establish a mouse model bearing human prostate cancer xenograft with bone metastasis by the monitoring with X-Ray,Micro CT,and 18F-NaF PET/CT.Methods Sixteen male Balb/c nude mice were allocated into control (6 mice) and experimental group (10 mice).In experimental group,the mice were subjected to percutaneous injection of 2 × 105 PC-3 cells into tibial plateau,bone defects were assessed after 21 d by X-ray,Micro-CT and 18F-NaF PET/CT,and bone damages were evaluated by HE staining.In control group,equal volume of saline was injected into the mice.Results At 21 d post modeling,the significant radioactive 18F-NaF signals were found in the tibial intramedullary cavity of all 10 mice in experimental group.The ROI value evaluation showed that SUVmax in control group was 0.62±0.14,but SUVmax in tumor group was 2.10±0.13,which indicated abnormal bone metabolism.The serum alkaline phosphate level and HE staining results also confirmed that tumor mediated bone destruction and osteogenesis.However,X ray and Micro-CT did not indicate precise diagnostic bone defect.Conclusion Bone metastasis model of prostate PC-3 cancer cells were successfully established by intratibial injection.18 F-NaF PET/CT could detect tumor invasion and bone osteogenesis in the early stage of modeling.关键词
前列腺癌/骨转移/18F-NaF PET-CTKey words
Prostate cancer/Bone metastasis/18F-NaF PET/CT引用本文复制引用
尤强,蔡华伟,庞富文,王哲涛,陈跃,田蓉,李林,陈利弘..18F-NaF PET-CT显像技术辅助下的前列腺癌骨转移动物模型建立[J].四川大学学报（医学版）,2017,48(2):276-281,6.基金项目
国家自然科学基金(No.81301250、No.81471693)和四川大学青年教师科研启动基金(No.2013SCU11002)资助 （No.81301250、No.81471693）
