四川大学学报（医学版）2017，Vol.48Issue(2)：295-298,4.
CFN-MPS-200模块自动化制备18F-FLT及其质量控制与生物评价
Quality Control and Biological Assessment of Automated Synthesized 3'-deoxy-3'-18F-fluorothymidine using CFN-MPS-200 Module
摘要
Abstract
Objective To synthesize 3'-deoxy-3'-18F-fluorotyhymidine (18F-FLT) using CFN-MPS-200 automatic synthesis module,and evaluate its distribution in Wistar rats.Methods We used 3-N-Boc-5'-O-dimethoxytrityl-3'-O-nosyl-thymidine (Boc-FLT)-percursor as raw material to synthesize 18F-FLT without residual solvents.Its radiochemical purity was confirmed with radio-HPLC and thin layer chromatography (TLC).Normal Wistar rats were injected with 18F-FLT and underwent PET scanning.Results The entire preparation procedure took about 60 min,which resulted in a radio chemical yield of (24 ± 5) ％ (after attenuation correction,n =20) and radiochemical purity of over 99％,with 1.11 × 109 Bq/mL specific activity.The 18F-FLT solution was colorless and had a pH value between 7.0-8.0.18F-FLT was mainly concentrated in the kidney,bladder,liver,bone marrow and Liver of normal Wistar rats.Conclusion Automated synthesis of 18F-FLT using CFN-MPS-200 is a stable method,with high yield,safety without solvent,and acceptable quality.关键词
18F-FLT/PET分子探针/放射性药物/自动合成/PET扫描Key words
18F-FLT/PET tracer/Radiopharmaceutical/Automated synthesis/PET scanning引用本文复制引用
江骁,程祝忠,王潇雄,陆皓,陈世容,肖定琼,赵檬,李秀丽,张歌,许国辉..CFN-MPS-200模块自动化制备18F-FLT及其质量控制与生物评价[J].四川大学学报（医学版）,2017,48(2):295-298,4.基金项目
四川省科技计划项目(No.2015JY0026)资助 （No.2015JY0026）
