海洋开发与管理2017，Vol.34Issue(2)：92-95,4.
DNA条形码技术在仔稚鱼鉴定中的实践
Molecular Identification of Fish Larvae and Juveniles Based on DNA Barcoding
摘要
Abstract
Accurate identification of species is a prerequisite for biodiversity research.To improve the identification accuracy of fish larvae and j uveniles in the Yangtze Estuary,the method of mor-phology and DNA barcoding were used based on mitochondrial the COI gene.The results showed that among the 44 samples analyzed,17 of which could be identified to the species level using DNA barcoding,while only one to the genus level.Some species without available taxonomic keys for morphological identification,including Chaeturichthys stigmatias,Acanthogobius ommaturus,Lo-phiogobius ocellicauda,Rhinogobius brunneus,Luciogobius guttatus in Gobiidae,were difficult to iden-tify by morphology but were successfully identified by using DNA barcoding.Some species with similar morphology were hard to be distinguished,such as Mylopharyngodon piceus,Hypophthalmichthys molitrix,Parabramis peknensis in Freshwater Cyprinidae,Chelon haematocheilus, ChelonaffinisinMugilidae.DNA barcoding based on the COI gene could provide effective identi-fication to the species level.Collectively,DNA barcoding,together with morphological identifica-tion,could largely increase the identification accuracy for fish larvae and j uveniles.关键词
DNA条形码/生物多样性/COI基因/海洋生物/物种鉴定Key words
DNA barcoding/Biodiversity/COI gene/Marine organism/Species identification分类
海洋科学引用本文复制引用
刘守海,秦玉涛,刘材材,季晓,张昊飞..DNA条形码技术在仔稚鱼鉴定中的实践[J].海洋开发与管理,2017,34(2):92-95,4.基金项目
海洋公益性行业科研专项(201305027,201505004-6) （201305027,201505004-6）
海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室基金资助课题(MATHAB201402,MATHAB201408) （MATHAB201402,MATHAB201408）
东海分局青年海洋科技基金项目(201501,201608). （201501,201608）
