基础医学与临床2017，Vol.37Issue(3)：341-345,5.
高胰岛素增加 早孕小鼠子宫内膜蜕膜化进程中血管紧张素Ⅱ的表达
High insulin increases expression of angiogenensis Ⅱin the endometrium of early pregnant mice
摘要
Abstract
Objective To investigate the effect of high level insulin on the vascular marker molecules Ang-Ⅱin the endometrium of early pregnant mice .Methods The high insulin animal model was constructed by subcutaneous in-jection of insulin .The uterine tissues and serum from D6 , D7 , and D8 days of pregnancy which were collected af-ter mating with normal male rats , and blood glucose was simultaneously monitored .In addition to the male rats with vasectomized mating to construct artificial decidualization .ELISA for the detection of serum insulin , progesterone , and estradiol changes , real time PCR to detect the Ang-ⅡmRNA expression in endometrial tissue .Western blot for the detection of Ang-Ⅱprotein expression in uterine tissue .Results Insulin in the high insulin group of pregnancy D6, D7, and D8 days increased significantly(P<0.001), estrogen levels in pregnancy D8 days appear significant-ly decreased ( P<0.05 ) and progesterone levels in pregnancy D 6 and D8 days appear to decline significantly ( P<0.01).The expression of Ang-ⅡmRNA and protein significantly increased in the high insulin model (P<0.05).Conclu-sions High insulin can increase the expression of Ang-Ⅱin the endometrium of pregnant rats during the process of preg-nancy.It is suggested that insulin may may contribute to the angiogenesis in the endometrium of the uterus .关键词
胰岛素/子宫内膜蜕膜化/Ang-Ⅱ/血管生成Key words
insulin/decidualization/Ang-Ⅱ/angiogenesis分类
医药卫生引用本文复制引用
陈文琪,张晨,王应雄,何俊琳,刘学庆,陈雪梅,穆欣艺,丁裕斌,高茹菲..高胰岛素增加 早孕小鼠子宫内膜蜕膜化进程中血管紧张素Ⅱ的表达[J].基础医学与临床,2017,37(3):341-345,5.基金项目
国家自然科学基金青年基金(81300486) （81300486）
重庆市科委前沿与应用基础研究项目(cstc2015jcyjA10013) （cstc2015jcyjA10013）
重庆市渝中区基础与前沿科技项目(20150104 ) （20150104 ）
