基础医学与临床2017，Vol.37Issue(3)：391-395,5.
尼古丁促人脐静脉内皮细胞凋亡
Nicotine promotes the apoptosis of human umbilical vein endothelial cells
刘秀 1邱宸阳 2王超楠 1刘暴2
作者信息
- 1. 中国医学科学院北京协和医学院北京协和医院血管外科,北京100730
- 2. 河北北方学院研究生部,河北张家口075000
- 折叠
摘要
Abstract
Objective To try different concentrations of nicotine promotes the apoptosis of human umbilical vein en-dothelial cells and its mechanism .Methods Human umbilical vein endothelial cells were cultured .According to the concentration of nicotine , grouped as 10 -6 , 10 -7, 10 -8 mol/L, and control.After 24 h of nicotine treat-ment, proliferation/activity were tested by CCK-8 assay;Annexin V-FITC fluorescence staining detects the apoptot-ic cells.Western blot detects the expression of protein Bax , Bcl-2 and PARP-1, to study the mechanism of apopto-sis.Results Compared with control group , in 10 -6 ,10 -7 mol/L groups the proliferation/activity of HUVECs de-creased ( P<0.05 ) .And the number of apoptotic cells increased ( P<0.01 ) .The target proteins expression of 10 -6、10 -7 mol/L groups were changed as follow: Bax increased ( P <0.01 ) , Bcl-2 decreased ( P <0.01 ) and PARP-1 increased ( P<0.01 ) .Conclusions Nicotine may promote the apoptosis of vascular endothelial cells , which accelerates the development of atherosclerotic disease .关键词
人脐静脉内皮细胞/尼古丁/凋亡Key words
human umbilical vein endothelial cells/nicotine/apoptosis分类
医药卫生引用本文复制引用
刘秀,邱宸阳,王超楠,刘暴..尼古丁促人脐静脉内皮细胞凋亡[J].基础医学与临床,2017,37(3):391-395,5.
