吉林中医药2017，Vol.37Issue(3)：297-300,4.DOI:10.13463/j.cnki.jlzyy.2017.03.023
桦褐孔菌系统溶剂法提取物体外抗肿瘤活性部位的筛选
Screening of antitumor active site in vitro extract of inonotus obliquus by system solvent
摘要
Abstract
Objective To screen out the main active site antitumor, comparing antitumor activity of different polarity organic reagent extractions of inonotus obliquus. Methods By using the system solvent method to gradient extraction sequence of inonotus obliquus,respectively get petroleum ether layer, chloroform layer, ethyl acetate layer, n-butyl alcohol layer, 70% alcohol layer, water layer and seven different polar parts such as coarse polysaccharide. As determined by MTT method to investigate inhibition of the polar parts of inonotus obliquus for liver cancer cells, lung cancer cells and inhibition of breast cancer cells. Results 7 kinds of extracts of inonotus obliquus all can inhibit cancer cell growth of concentration dependence. At the same time the chloroform layer have higher inhibition rate of the cancer cells. Conclusion Chloroform layer is the main antitumor activity of inonotus obliquus, which has high antitumor activity.关键词
桦褐孔菌/系统溶剂法/抗肿瘤活性Key words
inonotus obliquus/systematic solvent method/antitumor activity分类
医药卫生引用本文复制引用
季存蕊,杜延佳,孙佳明,张辉..桦褐孔菌系统溶剂法提取物体外抗肿瘤活性部位的筛选[J].吉林中医药,2017,37(3):297-300,4.基金项目
中医药行业科研专项(201207002-05). （201207002-05）
