江苏大学学报（医学版）2017，Vol.27Issue(1)：1-4,4.DOI:10.13312/j.issn.1671-7783.y160289
穴位埋线对心肌缺血再灌注过程自噬相关蛋白表达的影响
Expression of autophagy associated protein during myocardial ischemia reperfusion upregulated by acupoint catgut implantation
摘要
Abstract
Objective:To investigate the effect of acupoint catgut implantation on the expressions of pro-tein Beclin-1 ,Bcl-2 and P62 in myocardium of ZDF rats with ischemiareperfusion injury.Methods:Twentyfour male ZDF rats were randomly divided into four groups(n=6 ):blank group,ischemia-reperfu-sion group,acupuncture group and acupoint catgut implantation group.Myocardial ischemia-reperfusion in-jury model was prepared by push tube method.The levels of superoxide dismutase(SOD),glutathione per-oxidase (GSH-Px)were measured by ELISA method.The protein expressions of Beclin-1 ,Bcl-2 and P62 were detected by Western blotting.The ultrastructure of myocardium was observed by electron microscope. Results:Compared with the blank group,the levels of SOD and GSH-Px in the other three groups were sig-nificantly decreased(P<0.01),and the expression of Beclin-1,Bcl-2 and P62 were increased(P<0.01) .Compared with ischemiareperfusion group ,the levels of SOD ,GSH-Px in the acupuncture group and the catgut implantation group increased(P<0.01),the expression of Beclin-1 and Bcl-2 increased and the P62 protein decreased(P<0.01).Compared with the ischemia-reperfusion group,ZDF rats in the acupuncture group and the catgut implantation group had lower myocardial injury and increased number of autophagic vacuoles.Conclusion:Catgut implantation may protect myocardium against ischemia-reperfusion injury by activation of autophagy in myocardium.关键词
埋线/心肌/缺血再灌注/Beclin-1/P62/自噬Key words
catgutimplantation/myocardium/ischemiareperfusion/Beclin-1/P62/autophagy分类
医药卫生引用本文复制引用
王一茗,杨晓月,束彦页,万晓蕾..穴位埋线对心肌缺血再灌注过程自噬相关蛋白表达的影响[J].江苏大学学报（医学版）,2017,27(1):1-4,4.基金项目
国家自然科学基金资助项目(81303037) （81303037）
