江苏大学学报（医学版）2017，Vol.27Issue(1)：27-30,35,5.DOI:10.13312/j.issn.1671-7783.y160240
维生素E对帕金森病模型细胞的抗凋亡作用及其机制
Antiapoptotic effects and potential mechanism of Vitamin E on cellular model of Parkinson′s disease
摘要
Abstract
Objective:To explore the protective role of Vitamin E(Vit E)in 1-methyl-4-phenylpyridini-um (MPP+)induced neuronal apoptosis and the probable mechanism.Methods:Nerve growth factor (NGF)was used to induce PC12 cells differentiating to neurons.MPP+were added into culture medium to establish Parkinson′s disease(PD)cell model which then be added with different concentrations of Vit E to protect apoptotic neurons.Fluorescence microscope was used to detect the cellular morphology after Hoechst 33342 staining.The apoptotic and necrotic rate in each group was detected by FCM.Western blotting was used to detect the expression of CKD5 and calpains in each group PC12 cells.Results:Cells clustered and had good refractive power under an inverted microscope in the 2nd day after NGF added.Nuclear pyknosis and fragmentation was least in 200 μmol/L concentration of Vit E under fluorescence microscope.FCM showed cell viability elevated (P<0.01 )and apoptotic rate declined (P<0.01 )with increasing of con-centration of Vit E.Cell viability reached the peak (P<0.01)and apoptotic rate reduced to the bottom (P<0.01)in 200 μmol/L concentration of Vit E.Expressions of CKD5 and calpains decreased with increasing concentration of Vit E.Conclusion:Vit E plays an important role in neuronal protection by inhibiting calpains pathways in PD.关键词
维生素E/帕金森病/细胞凋亡/calpainsKey words
VitaminE/Parkinson′sdisease/cellapoptosis/calpains分类
医药卫生引用本文复制引用
杨溢,何悦,陈思远,于明..维生素E对帕金森病模型细胞的抗凋亡作用及其机制[J].江苏大学学报（医学版）,2017,27(1):27-30,35,5.基金项目
镇江市社会发展项目(SH2015024 （）
SH2015047) （）
江苏省"333工程"资助项目(BRA2014173) （BRA2014173）
江苏省"六大人才高峰"资助项目(WSN038) （WSN038）
