扬州大学学报（农业与生命科学版）2016，Vol.37Issue(4)：5-8,4.DOI:10.16872/j.cnki.1671-4652.2016.04.002
肠炎沙门菌调控基因hilD与hilA的体外表达规律研究
Analysis of the in vitro expression characteristics of regulatory genes hilD and hilA from Salmonella enterica serovar Enteritidis
摘要
Abstract
The prokaryotic expression plasmids pCold Ⅰ was used to construct recombinant expression bacteria expressing two regulators hilD and hilA from Salmonella enterica serovar Enteritidis,and western blot analysis was applied in detecting the expression pattern of both regulators under the culture condition of LB medium.The result showed that the recombinant expression bacteria E.coli BL21(pCold Ⅰ-hilD) and E.coli BL21(pCold Ⅰ-hilA) was successfully constructed.When the bacteria were induced by IPTG,the recombinant proteins were collected,purified,and then inoculated into BALB/c to induce the production of polyclonal antibodies,which were identified by western blot analysis.The Western blot result revealed the different expression pattern of both regulators in vitro,which could be used to study the function of effector proteins belonging to SPI1 and SPI2 in vitro.关键词
肠炎沙门菌/HilA/HilD/Western blotKey words
Salmonella enterica serovar Enteritidis/HilA/HilD/Western blot分类
医药卫生引用本文复制引用
刘子健,潘志明,焦新安,陈韵,李扬,尹克全,徐黎娟,尹超,夏颉,谢晓蕾,李求春..肠炎沙门菌调控基因hilD与hilA的体外表达规律研究[J].扬州大学学报（农业与生命科学版）,2016,37(4):5-8,4.基金项目
国家自然科学基金资助项目(31320103907、31230070) （31320103907、31230070）
公益性行业(农业)科研专项(201403054) （农业）
“十二五”国家科技支撑计划项目(2014BAD13B02) （2014BAD13B02）
江苏省高校优势学科建设工程项目(PADD) （PADD）
