扬州大学学报（农业与生命科学版）2016，Vol.37Issue(4)：27-31,48,6.DOI:10.16872/j.cnki.1671-4652.2016.04.006
富硒女贞子对热应激卵巢细胞的影响
Influence of Se-enriched ligustri lucidi fructus on ovarian cells under heat stress
摘要
Abstract
In the experiment,the heat stress model temperature was set as 42 ℃ and the temperature 37 ℃ was set as the control,heat-stressed ovarian cells were cultured with different concentration of water extract,polysaccharides,protein and oleanolic acid extract of Se-LLF.The growth and apoptosis rate of cultured ovarian cells were respectively detected by MTT and flow cytometry,and the content of SOD in the supernatant fluid of cultured ovarian cells was detected by xanthine oxidase to investigate the effects of se-enriched Ligustri Lucidi Fructus on proliferation,apoptosis and antioxidant activity of ovarian cells cultured under heat circumstance in vitro.The results showed that the proliferation of ovarian cells cultured under high temperature was significantly promoted in each experimental concentration of water extract and polysaccharides extract of Se-LLF (P＜0.05).The apoptosis rate decreased significantly (P＜0.05),the activity of SOD was increased significantly (P＜0.05),and the results were better than those of the ordinary Ligustrum Lucidum group.In conclusion,water and polysaccharides extract of Se-LLF can not only improve the proliferation and antioxidant capacity of heat-stressed ovarian cells,but also can inhibit their apoptosis.关键词
热应激/富硒女贞子/凋亡/卵巢细胞Key words
heat stress/Se-enriched ligustri lucidi fructus/apoptosis/ovarian cells分类
农业科技引用本文复制引用
邹慧,李丹丹,陈静,劳雪芬,曹铮,尚秋辰,许小琴..富硒女贞子对热应激卵巢细胞的影响[J].扬州大学学报（农业与生命科学版）,2016,37(4):27-31,48,6.基金项目
国家自然科学基金资助项目(31172354) （31172354）
江苏省高校优势学科建设工程资助项目(2013-03) （2013-03）
