扬州大学学报（农业与生命科学版）2016，Vol.37Issue(4)：55-59,102,6.DOI:10.16872/j.cnki.1671-4652.2016.04.011
水稻粒长基因qGL3功能标记的开发与鉴定
Development and identification of functional marker for rice grain length gene qGL3
摘要
Abstract
Based on the analysis of genomic DNA sequence and PCR,the genotypes of grain length gene qGL3 were identified in sequenced wild species of genus Oryza,294 accessions of rice mini core collection (RMCC) and 65 japonica varieties certificated in Jiangsu Province from 2007 to 2013.Homologous sequences of gene qGL3 were found in all the wild rices involved.The phylogeny based on the coding sequence of gene qGL3 demonstrated an apparent differentiation among the wild species of genus Oryza.Subsequently one convenient functional dCAPS marker was developed on the A/C missense mutation in the exon10 of gene qGL3 and used to identify the genotypes of the RMCC.Genotype qgl3 represented base A on the mutant sites appeared only in one variety,which is a rare allele.But genotype qGL3 represented base C on the target site occurred in 293 varieties,which is a prevalent allele.Meanwhile the qGL3 genotype was found in all the 65 japonica varieties certificated in Jiangsu Provice.关键词
水稻/粒长/qGL3基因/功能标记Key words
rice/grain length/qGL3 gene/functional marker分类
农业科技引用本文复制引用
裔传灯,李玮,王德荣,蒋伟,薛松,耿金鹏,周勇,梁国华..水稻粒长基因qGL3功能标记的开发与鉴定[J].扬州大学学报（农业与生命科学版）,2016,37(4):55-59,102,6.基金项目
国家自然科学基金资助项目(31571624、31071382) （31571624、31071382）
国家重点基础研究发展计划项目(973-2010CB125904、973-2013CBA01405) （973-2010CB125904、973-2013CBA01405）
江苏省高校自然科学研究重大项目(15KJA210004) （15KJA210004）
江苏省高校优势学科建设工程项目(2014-12) （2014-12）
