军事医学2017，Vol.41Issue(1)：33-37,5.DOI:10.7644/j.issn.1674-9960.2017.01.008
可溶性Tim-3体外增强免疫应答的作用及机制研究
Promotion of immune response by soluble Tim-3 in vitro and therapeutic potential
摘要
Abstract
Objective To evaluate the role of recombinant human soluble Tim-3 (hTim-3-Fc) in regulating immune response.Methods Soluble hTim-3 was incubated with human macrophage cell line U 937, human T cell line Jurkat and normal human PBMC before cytokines secreted by or expressed in different immune cells were analyzed using ELISA , RT-PCR and Western-blotting, respectively.Results Soluble hTim-3 significantly promoted the activation of different immune cells.Our data showed that IL-8 secretion by U937 cells, IL-2 secretion by Jurkat cells , IL-2 and IFN-γsecretion by human PBMCs were all significantly increased .In addition , soluble hTim-3 significantly increased the IFN-α2 and IFN-β1 mRNA expression in U937, Jurkat and PBMCs and increased the phosphorylation of stat-1 in Jurkat and U937 cells.Conclusion Recombinant soluble hTim-3 can significantly promote the activation of immune cells in vitro, which shows its therapeutic potential .关键词
Tim-3/融合蛋白/细胞因子类/U937细胞/Jurkat细胞/T淋巴细胞Key words
Tim-3/fusion protein/cytokines/U937 cells/Jurkat cells/T-lymphocytes分类
医药卫生引用本文复制引用
李葛,王智鼎,窦帅杰,侯春梅,肖鹤,王仁喜,陈国江,黎燕,韩根成..可溶性Tim-3体外增强免疫应答的作用及机制研究[J].军事医学,2017,41(1):33-37,5.基金项目
国家自然科学基金面上资助项目（81471540）；北京市自然科学基金重点资助项目 （）
