军事医学2017，Vol.41Issue(1)：43-47,61,6.DOI:10.7644/j.issn.1674-9960.2017.01.010
RNF34调控天然免疫的初步研究
Mechanism of RING finger protein 34 involved in innate immunity:a preliminary study
摘要
Abstract
Objective To study the mechanism of RING finger protein 34 ( RNF34 ) involved in innate immunity . Methods Recombinant PCR was used and transient expression of the plasmid was achieved in HEK 293T cells.The cells were stimulated with Sendai virus ( SeV) or N-RIG-Ⅰfor the indicated time while luciferase activity was observed using the dual-luciferase reporter assay kit .Results We constructed the plasmid pcDNA 3-Flag-RNF34 and its three mutations .The study found that when stimulated by SeV , RNF34 could inhibit the activity of NF-κB and IFN-βmore significantly than RNF34-ΔFYVE, RNF34-ΔCID and RNF34-ΔRING.We also found that RNF 34 and its three mutants had similar inhibitory effect when the activation of NF-κB and IFN-βwas stimulated by the N-RIG-Ⅰ.Conclusion RNF34 negatively regulates innate immunity by acting on the RIG-Ⅰ-MAVS signaling pathway .关键词
RNF34/免疫,天然/RIG-Ⅰ/NF-κB/干扰素βKey words
RNF34/innate immunity/RIG-Ⅰ/NF-κB/IFN-β分类
医药卫生引用本文复制引用
朱永杰,张萍萍,周鹏宇,王鹏皓,陈健康,田茵茵,何湘,钟辉..RNF34调控天然免疫的初步研究[J].军事医学,2017,41(1):43-47,61,6.基金项目
国家自然科学基金资助项目 （）
