军事医学2017，Vol.41Issue(1)：53-57,5.DOI:10.7644/j.issn.1674-9960.2017.01.012
B型流感病毒小鼠致死模型的建立
Establishment of a lethal mouse model of influenza B virus
摘要
Abstract
Objective To establish a mouse lethal model of influenza B virus , which will facilitate the study on the mechanism of pathogenesis , transmission of influenza B virus , development of new vaccines and drugs against influenza B virus.Methods We obtained a mouse adaptive B/Lee/1940 virus by continuously passaging it in mice for 5 cycles.The P5 virus was propagated in MDCK cells , which was used for infecting mice .The body mass and survival rate of mice were monitored during the following 14 days after infection.At the same time,the 8 gene segments (PB2, PB1, PA, HA, NA/NB, NP, M, and NS) of P0 and P5 virus were sequenced and analyzed .Results and Conclusion Virus was detected in the lungs of mice in each generation in the process of virus passaging .The body mass of mice infected with the deadly mouse adaptive virus changed dramatically .The mortality of mice was 100%, and virus was detected in mouse lungs . Sequence analysis results indicated that the amino acid mutations occurred in PB 2 and NP.A series of experiments indicated that we had established a mouse lethal model of influenza B virus .关键词
流感病毒,B型/模型,动物/BALB/c小鼠/致死毒株/连续传代/死亡率Key words
influenza B virus/models, animal/BALB/c mouse/lethal virus/serial passage/mortality分类
医药卫生引用本文复制引用
张海亮,冯俊霞,肖冬光,校海霞..B型流感病毒小鼠致死模型的建立[J].军事医学,2017,41(1):53-57,5.基金项目
中国科学院科技攻关项目 （）
