军事医学2017，Vol.41Issue(2)：90-95,6.DOI:10.7644/j.issn.1674-9960.2017.02.003
SIRT1对肝癌细胞耐药敏感性的影响
Effect of SIRT1 on drug resistance of hepatocellular carcinoma cells
摘要
Abstract
Objective To build a short-hairpin RNA (shRNA)interference lentiviral vector of silent information regulator(sir)-like protein 1 (Sirtuin 1,SIRT1 )gene and to explore its effect on proliferation and drug resistance in hepatocellular carcinoma(HCC)cells.Methods The SIRI1 target specific oligonucleotide sequence was designed and connected to pSicoR-green fluorescent protein(GFP)vector digested by the HpaⅠ and XhoⅠ.Then,293T cells were packaged to produce the lentivirus and transfected with liver cancer cells.qRT-PCR assays further indicated that SIRT1 gene was stably knocked down in HCC cells.The effect on cell proliferation was detected by foci formation assay and CCK8 assay.In addition,drug resistance experiments and qRT-PCR were used to detect the effect on resistance of liver cancer cells when the SIRT1 gene was knocked down.Results The result of qRT-PCR demonstrated that the lentivirus shRNA vector of SIRT1 gene was constructed and could suppress the expression level of SIRT1.The proliferation and drug resistance genes of HCC cells were inhibited and drug sensitivity was enhanced.Conclusion SIRT1 gene represses the drug resistance of HCC cells.关键词
沉默信息调节因子样蛋白1/RNA干扰/基因敲除/肝癌/耐药/间充质干细胞/聚合酶链反应Key words
silent information regulator sirtuin 1/RNA interference/geno knockout/hepatocellular carcinoma/drug-resistance/mesenchymal stem cells/polymerase chain reaction分类
医药卫生引用本文复制引用
纪光红,阎新龙,王海洋,张彪,曾泉,周军年,范增,黄映辉,岳文..SIRT1对肝癌细胞耐药敏感性的影响[J].军事医学,2017,41(2):90-95,6.基金项目
国家863计划资助项目(2013AA020107) （2013AA020107）
广州市健康医疗协同创新重大专项资助项目(201400000003-1) （201400000003-1）
国家自然科学基金资助项目(81472341) （81472341）
北京市自然科学基金资助项目(7152095) （7152095）
