军事医学2017，Vol.41Issue(2)：101-105,5.DOI:10.7644/j.issn.1674-9960.2017.02.005
广西1例HIV-1 G亚型毒株全长基因序列的测定和分析
Near full-length genomic analysis of an HIV-1 G subtype strain identified in Guangxi Zhuang Autonomous Region
摘要
Abstract
Objective To sequence and analyze the near full-length genome of an HIV-1 subtype G strain identified in Guangxi,China.Methods The demographic information of an individual infected with HIV-1 subtype G strain was investigated,whose peripherial blood was collected.Viral RNA in plasma was extracted.The near full-length genome of HIV was amplified in two halves using RT-nested-PCR.The PCR products were purified and sequenced.Phylogenetic analysis was made using MEGA6 software.Results A near full-length genome of 8847 bp was obtained.In the neighbor-joining tree,the strain clustered with subtype G references,as supported by the high Bootstrap value (100%).The closest phylogenetic relationship was found between our strain and another subtype G strain (JN106043)previously identified in Guangxi,which was supported by the genetic distance (5%)and high Bootstrap value (100%).Conclusion The strain identified in the study might have originated from subtype G strains in Guangxi,suggesting that subtype G has spread locally in Guangxi.Further surveillance of subtype G epidemic in Guangxi is necessary.The near full-length subtype G strain genome will provide information for the surveillance of HIV in Guangxi.关键词
HIV-1/G亚型/近似全长序列/系统进化树分析Key words
HIV-1/G-subtype/near full-length sequence/phylogenetic tree analysis分类
医药卫生引用本文复制引用
张敏,梁冰玉,李天一,沈平,苏齐鉴,李敬云,李林..广西1例HIV-1 G亚型毒株全长基因序列的测定和分析[J].军事医学,2017,41(2):101-105,5.基金项目
国家自然科学基金资助项目(81273137) （81273137）
