军事医学2017，Vol.41Issue(2)：106-110,5.DOI:10.7644/j.issn.1674-9960.2017.02.006
皮质抑素在人肾组织中及IgA肾病肾组织中的表达情况
Cortistatin expression in renal tissue of humans and IgA nephropathy patients
摘要
Abstract
Objective To explore the condition of cortistatin (CST)expression in human renal tissue and the changes in the level of CST in IgA nephropathy (IgAN)of different degrees.Methods Ten tumor adjacent normal renal tissue samples were collected.The mRNA and protein expressions of CST in human renal tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting,respectively.Immunohistochemisty (IHC)was performed to locate the expression of CST in renal tissue.According to the grading system of Lee et al,IgAN was divided into three groups:grade Ⅰ -Ⅱ (group A),grade Ⅲ -Ⅳ (group B),and grade Ⅴ (group C),and ten renal biopsy tissue samples were collected for each group.IHC was performed to detect the change in the level of CST in normal and IgAN renal tissue of different degrees.The effect of clinical indices on the level of CST in IgAN renal tissue was assessed by multiple linear regression analysis.Results RT-PCR and Western blotting showed that CST was expressed in renal tissue and IHC showed that CST was expressed on renal tubular epithelial cells.In IgAN,the higher the pathological grade was, the higher the expression of CST in renal tubules was.Multiple linear regression analysis showed that the pathological grade was associated with the expression of CST in renal tissue (r =0.875,P <0.01).Conclusion CST may participate in the inflammatory reaction of IgAN pathological injury and exert anti-inflammation effects.关键词
皮质抑素/肾组织/IgA肾病Key words
cortistatin/renal tissue/IgA nephropathy分类
医药卫生引用本文复制引用
宁小元,陈霞,焦晓静,张雪光,徐家云..皮质抑素在人肾组织中及IgA肾病肾组织中的表达情况[J].军事医学,2017,41(2):106-110,5.基金项目
国家基础研究资助项目(2014CBA02005) （2014CBA02005）
