江西农业大学学报2017，Vol.39Issue(1)：37-42,6.DOI:10.13836/j.jjau.2017005
铝胁迫对烟草叶片呼吸作用和活性氧代谢的影响
Effects of Aluminum Stress on Respiration and Reactive Oxygen Metabolism in Leaves of Tobacco Seedlings
摘要
Abstract
Two different aluminum ( Al)-tolerant tobacco cultivars Yunyan 100 ( Al-tolerant) and Yunyan 105 (Al-sensitive) were used to study the effects of four Al concentrations (0,50,100,200μmol/L) on plant biomass,leaf reactive oxygen content and respiration,activities of antioxidant enzymes in mitochondria.The re-sults showed that the plant biomass decreased significantly,and the leaf O·-2 and H2 O2 contents increased sig-nificantly with the increase of Al concentrations,which was more obvious in Al-sensitive cv. Yunyan 105,sug-gesting that Al phytotoxicity was positively related to leaf reactive oxygen ( ROS) accumulation. With the Al concentrations increased from 0 to 200 μmol/L,leaf total respiration,cytochrome respiration and cytochrome c oxidase ( COX) activity decreased steadly,while alternative respiration and pyruvate content were promoted firstly and then reduced again,indicating that inhibition of COX activity and promotion of pyruvate contentexplained the changes in different pathway respiration in tobacco under Al stress. Meanwhile,Al significantly increased the activities of superoxide dismutase ( SOD) and ascobate peroxidase ( APX) in mitochondria,con-tributing to elimination of intracellular ROS.Compared with Al-sensitive cv.Yunyan 105,Al-tolerant cv.Yunyan 100 remained higher alternative respiration,total respiration,activities of SOD and APX in mitochondria under different Al concentrations.These results indicated that higher Al tolerance of tobacco is associated with the ca-pacity to keep high respiration,espcially alternative respiration,and activities of SOD and APX in mitochondria under Al stress.关键词
铝胁迫/活性氧/呼吸作用/抗氧化酶/烟草Key words
aluminum stress/reactive oxygen species/respiration/antioxidant enzyme/tobacco分类
农业科技引用本文复制引用
刘强,柳正葳,贺根和,胡萃..铝胁迫对烟草叶片呼吸作用和活性氧代谢的影响[J].江西农业大学学报,2017,39(1):37-42,6.基金项目
国家自然科学基金项目( 31260498)、江西省自然科学基金项目( 2010GQN0122)和江西省教育厅科技项目( GJJ12466) Project supported by the National Natural Science Foundation of China (31260498),Natural Science Foundation of Jiangxi Province(2010GQN0122) and Scientific Program of Education Department of Jiangxi Province (GJJ12466) （ 31260498）
