江西农业大学学报2017，Vol.39Issue(1)：168-174,7.DOI:10.13836/j.jjau.2017022
苋菜amaAG基因克隆与生物信息学分析
Cloning and Bioinformatics Analysis of amaAG in Amaranthus tricolor L.
摘要
Abstract
The seeds were used to culture in vitro plantlets of Amaranthus tricolor as the material. The full-length amaAG cDNA sequence was cloned using transcriptome database of A. tricolor ( NCBI SRA number:SRR924089,SSR924090,SRR924091,SRR924092) ,RT-PCR and RACE techniques,and the bioinformatics was analyzed. The full-length amaAG cDNA sequence was 1123 bp in length,containing an open reading frame of 741 bp ( encoding 246 animo acids) ,flanked by 122 bp 5′ UTR and 243 bp 3′UTR that contained a 17 bp polyA. Bioinformatics analysis showed that the protein was hydrophilic cyto-plasmic protein,but not stable protein. The protein belongs to MADS and K-box superfamily with MADS_MEF2-like and K-box functional sites and the two sites were related to the growth of plants. The analysis of amaAG gene differential expression indicated that the gene expression level was the highest during flower-ing,which showed that the gene was cloned. The study is helpful for further research of amaAG functionand provides references for flower molecular mechanism of Amaranthus tricolor.关键词
苋菜/AG基因/克隆/开花/生物信息学Key words
Amaranthus tricolor L./amaAG/cloning/flower/bioinformatics分类
农业科技引用本文复制引用
柳燕,谢礼洋,赖钟雄,刘生财..苋菜amaAG基因克隆与生物信息学分析[J].江西农业大学学报,2017,39(1):168-174,7.基金项目
福建省重大科技专项(2015NZ0002)、高等学校博士学科点专项新教师类科研基金(20123515120009)和福建省自然科学基金( 2013J05045) Project supported by National Natural Science Foundation of China:Fujian Major Scientific and Technological Project(2015NZ0002),the Specialized Research Fund for the Doctoral Program New Teacher Kind of Higher Ed-ucation( 20123515120009) and Fujian Natural Science Fund( 2013J05045) （2015NZ0002）
