江西农业大学学报2017，Vol.39Issue(1)：182-189,8.DOI:10.13836/j.jjau.2017024
大孔树脂纯化桃花总黄酮工艺及抗氧化性研究
A Study on Purification of Total Flavonoids from Prunus persica by Macroporous Resin and Their Antioxidant Activity
摘要
Abstract
The conditions for purification of total flavonoids from Prunus persica by macroporous resin were explored.With adsorption-desorption ratios as indexes,optimum resin DM-28 was selected by static ad-sorption-desorption and dynamic adsorption- desorption ratio tests.The concentration of eluting agent was de-termined with the content of total flavonoids as the index. The optimum conditions for static absorption was as follows:the best temperature was 30 ℃,the value of pH 4.0,the time of absorption 3.5 h;the optimum condi-tion for dynamic adsorption was as follows: the flow rate 2 mL/min,sample concentration 0.72 mg/mL,and 60％ alcohol as eluant for desorption with the flow rate 2 mL/min. Under these conditions,the total flavonoid from Prunus persica increased by 6.84 times.Moreover,the antioxidant activity of flavonoids from Prunus persicawas explored by using free radical scavenging reactions.The results indicated that Prunus persica flavonoid has an obvious antioxidant activity,the IC50 value of DPPH and ABTS+ were 13.47μg/mL,32.16 μg/mL,respec-tively.关键词
桃花/总黄酮/大孔树脂/纯化/抗氧化性Key words
Prunus persica/total flavonoids/macroporous resins/purification/antioxidant activity分类
轻工纺织引用本文复制引用
曾臻,刘后根,洪艳平,吴国强,陆庆,许小向,胡洁芳,王学渊..大孔树脂纯化桃花总黄酮工艺及抗氧化性研究[J].江西农业大学学报,2017,39(1):182-189,8.基金项目
赣鄱英才"555"工程领军人才培养计划和江西农业大学大学生创新创业计划Project supported by the"555" Talents Program of Ganpo Excellence and the Inovation Program of Jiangxi Agri-cultural University （）
