江西农业学报2017，Vol.29Issue(3)：18-21,4.DOI:10.19386/j.cnki.jxnyxb.2017.03.04
水稻HGO基因启动子的克隆及转化鉴定
Cloning, Transformation and Identification of Gene HGO Promoter in Rice
摘要
Abstract
Gene HGO encodes 1,2-homogentisate dioxygenase, which functions in Tyr degradation pathway.In order to further investigate the expression characteristics of the HGO gene in rice, we constructed rice gene HGO promoter and GUS recombinant expression vector, and transformed this expression vector into Arabidopsis thaliana to obtain transgenic plants by using Agrobacterium-mediated floral dip method.By histochemical GUS staining, we found that the rice HGO gene promoter was highly expressed in the root, hypocotyl, petiole and cotyledon of A.thaliana, but it was lowly expressed in the euphylla vein and euphylla apex of A.thaliana.关键词
水稻/HGO基因/启动子/载体构建Key words
Rice/HGO gene/Promoter/Vector construction分类
生物科学引用本文复制引用
蔡薇,陈彦成,黄丽华,支添添,任春梅..水稻HGO基因启动子的克隆及转化鉴定[J].江西农业学报,2017,29(3):18-21,4.基金项目
国家科技部973前期研究专项(2014CB160308). （2014CB160308）
