解放军医学院学报2017，Vol.38Issue(1)：52-54,58,4.DOI:10.3969/j.issn.2095-5227.2017.01.014
跨膜蛋白106A在肝癌HuH7细胞中的功能研究
Role of TMEM106A in HuH7 cells
张浩赟 1吕文平 1夏艳 2严力 1王鹏飞 1徐凯 1陈永亮1
作者信息
- 1. 解放军医学院,北京 100853
- 2. 北京大学基础医学院 免疫系,北京 100191
- 折叠
摘要
Abstract
Objective To investigate the function of TMEM106A in Huh7 cells.Methods The expression of TMEM106A inHuH7 cells transfected with pcDB or pcDB-TMEM106A for 24 h were detected by Western blot, and the changes of cell morphology were observed under light microscopy. The vacuoles were labled with Neutral Red. The effects of TMEM106A on the activity of p38 MAPK were measured by Western blot.Results Overexpression of TMEM106A in Huh7 cells induced large vacuoles in cytoplasm, and the vacuoles could be dyed red by Neutral Red Staining. These vacuoles were originated from the perinuclear region. Western blot showed that the overexpression of TMEM106A had no effect on the activity of p38 MAPK.Conclusion Overexpression of TMEM106A in HuH7 cells induces large vacuoles. These vacuoles are acidic and they may come from endosome or lysosome.关键词
跨膜蛋白106A/胞质空泡化/p38促分裂原活化的蛋白激酶Key words
transmembrane protein 106A/cytoplasmic vacuolization/p38 MAPK分类
医药卫生引用本文复制引用
张浩赟,吕文平,夏艳,严力,王鹏飞,徐凯,陈永亮..跨膜蛋白106A在肝癌HuH7细胞中的功能研究[J].解放军医学院学报,2017,38(1):52-54,58,4.
