解放军医学院学报2017，Vol.38Issue(1)：55-58,4.DOI:10.3969/j.issn.2095-5227.2017.01.015
大鼠感觉、运动神经瓦勒氏变性前后蛋白分析
Protein expression profiling during wallerian degeneration in sensory and motor nerve in rat
摘要
Abstract
Objective Isobaric tags for relative and absolute quantitation (iTRAQ) and online two-dimensional liquid chromatographytandem mass spectrometry (2D LC-MS/MS) were performed to identify differentially expressed proteins in sensory and motor nerves at 7 days after transaction.Methods Forty adult male wistar rats weighing 220-250 g were randomly divided into control group and experimental group (n=20 each group). Spinal cord transaction was performed to rats in experimental group. Seven days later, samples of normal/injured ventral root nerve and dorsal root nerve were obtained and labeled using iTRAQ (114, 115, 116, 117 were used for labeling normal motor nerve, injured motor nerve, normal sensory nerve, injured motor nerve respectively), and analyzed by 2DLC-MS/MS.Results A total of 4312 proteins were identified. 1)There were 637 differentially expressed proteins in the injured motor nerve compared to the normal motor nerve, in which 265 were up-regulated and 372 were down-regulated. 2)There were 626 differentially expressed proteins in the injured sensory nerve compared to the normal sensory nerve, in which 258 were up-regulated and 368 were down-regulated proteins.Conclusion We screen out differentially expressed proteins in sensory and motor nerve in the process of Wallerian degeneration, and this may contribute to the research in peripheral nerve regeneration.关键词
周围神经损伤/瓦勒氏变性/同位素标记相对和绝对定量技术/二维液相色谱-串联质谱技术Key words
peripheral nerve injury/Wallerian degeneration/isobaric tags for relative and absolute quantitation/two-dimensional liquid chromatography-tandem mass spectrometry分类
医药卫生引用本文复制引用
苌彪,郭志远,全琦,孙逊,刘若西,鲁长风,王玉,汪爱媛,郭全义..大鼠感觉、运动神经瓦勒氏变性前后蛋白分析[J].解放军医学院学报,2017,38(1):55-58,4.基金项目
国家自然科学基金项目(51073024 （）
51273021) （）
973重点项目(2014CB542201 （）
2012CB518106) （）
军队"十三五"医学专项(BWS13C029) Supported by the National Natural Science Foundation of China (51073024 （BWS13C029）
51273021) （）
973 Program (2014CB542201 （）
2012CB518106) （）
the Special Project of the "Thirteenth Five-year Plan" for medical Science Development of PLA(BWS13C029) （BWS13C029）
