昆明医科大学学报2016，Vol.37Issue(8)：47-50,4.
帕瑞昔布钠预先给药对大鼠局灶脑缺血再灌注血脑屏障通透性的影响
Effect of Parecoxib Sodium Pretreatment on Permeability of Blood-Brain Barrier in a Rat Model of Focal Cerebra1 Ischemia-reperfusion Injury
摘要
Abstract
Objective To investigate the effect and the mechanism of parecoxib sodium pretreatment on permeability of blood-brain barrier in a rat model of focal cerebral ischemia-reperfusion injury.Methods Sixty male SD rats weighing 300g were randomly divided into 5 groups (n=12 each):sham operation group (group S);focal cerebral I/R group (group I/R);parecoxib sodium 5 mg/kg pretreatment group (group L);parecoxib sodium7.5mg/kg pretreatment group (group M);parecoxib sodium 10 mg/kg pretreatment group (group H) Middle cerebral artery occlusion models were made by reforming Longa suture method in SD rats.Thirty minutes before ischemia,rats in group L,M and H were injected with 5 mg/kg、7.5 mg/kg and 10 mg/kg parecoxib sodium through the internal jugular vein.Group S and group I/R received equal volume of normal saline.ELISA technique was used to determine the content of S100 β,TNF-α,IL-1 β in Plasma.The changes of cerebral water content and the Evans Blue exudation from brain capillaries were observed.Results Pretreated with parecoxib sodium (5mg/kg、7.5 mg/kg and 10 mg/kg),the content of S100 β,TNF-α,II-1 β in plasma were reduced.The cerebral water content and the EB in brain were reduced.Pretreated with parecoxib sodium 10 mg/kg,Longa scores were reduced.Conclusion Pretreatment with Parecoxib can protect blood-brain barrier against focal cerebral I/R injury by inhibition of the inflammatory reaetion.关键词
帕瑞昔布/脑缺血再灌注损伤/血脑屏障/细胞因子Key words
Parecoxib/Cerebral ischemia reperfusion injury/Blood-brain barrier/Cytokines分类
医药卫生引用本文复制引用
刘少星,谢先丰,曹德钧,杨孟昌,代月娥..帕瑞昔布钠预先给药对大鼠局灶脑缺血再灌注血脑屏障通透性的影响[J].昆明医科大学学报,2016,37(8):47-50,4.基金项目
四川省卫生厅科研基金资助项目(100520) （100520）
