昆明医科大学学报2016，Vol.37Issue(10)：5-9,5.
缺氧后不同复氧时间对心肌细胞自噬的影响
Effect of Different Reoxygenation Time on the Autophagy of Myocardial Cells
摘要
Abstract
Objective To investigate the effect of different time of hypoxia reoxygenation (HR) on cell autophagy and cell viability.Methods According to the different reoxygenation time,H9C2 cells were divided into five groups:normal control group (group A),hypoxia group (group B),reoxygen 2h group (group C),reoxygen 12h group (group D) and reoxygen 24h group (group E).The expression of LC3 Ⅱ/LC3 Ⅰ in each groups was detected by Western Blot,and the activity of myocardial cells was detected by MlT.Results MlT results showed that the viability of the myocardial cells after hypoxia 2h/reoxygenation 2h was significantly lower than that in the normal control group (P＜0.05).The ratio ofLC3 Ⅱ/LC3 Ⅰ of the cells in group B (hypoxia group),group C (reoxygen 2h group),group D (reoxygen 12h group),group E (reoxygen 24h group) was significantly higher than that of group A (normal control group) (P＜0.05).Among them,the ratio of LC3 Ⅱ/LC3 Ⅰ of the cells in group C and D were significantly higher than that of group B (P＜0.05),paticlularly the group D was the highest (P＜0.05).The activity of myocardial cells in group D was the lowest among all groups.Conclusion Culturing in the three gas incubator can obviously decrease the viability of H9C2cells.Hypoxia can activate autophagy,and autophagy increases significantly after reoxygenation.Autophagy may be activated most obviously at 12 hours and decrease to the level of hypoxia group at 24 hours.Cells can be damaged by hypoxia,and further increase of cellular damage may occur after oxygen inhalation.At 12 hours,the cell viability is the lowest,and the cell viability may be improved at 24h.关键词
自噬/大鼠心肌细胞/缺氧复氧Key words
Autophagy/Rat cardiomyocytes/Hypoxia/Reoxygenation分类
医药卫生引用本文复制引用
罗云燕,胡钊,肖践明,蔡红雁,李琳,郭涛..缺氧后不同复氧时间对心肌细胞自噬的影响[J].昆明医科大学学报,2016,37(10):5-9,5.基金项目
国家自然科学基金资助项目(81560072) （81560072）
云南省科技厅-昆明医科大学联合专项基金资助项目(2013FB128,2014FB037) （2013FB128,2014FB037）
昆明医科大学2015年硕士研究生创新项目(2015S83) （2015S83）
