林产化学与工业2017，Vol.37Issue(1)：94-100,7.DOI:10.3969/j.issn.0253-2417.2017.01.012
漆树木粉抗氧化活性成分的HPLC-MS分析
HPLC-MS Analysis of Antioxidant Components fromRhus verniciflua Stokes Wood Powder
摘要
Abstract
The ethanol extract from Rhus verniciflua Stokes wood powder was partitioned with different polarity solvents to obtain petroleum ether fraction, ethyl acetate fraction, n-butylalcohol fraction and water fraction.The DPPH·, ABTS· and OH· scavenging activities of the four fractions were evaluated.The results showed that the extraction yield of ethyl acetate was the highest (60.1%) and its scavenging activities of DPPH·, ABTS· and OH· were the strangest with the IC50 of 19.9, 29.74 and 37.95 mg/L, respectively.Twelve compounds in ethyl acetate fraction were identified by HPLC-MS, including six phenolic acids, i.e., ethoxy-3-hydroxy benzoic acid(1), gallic acid(2), 3,4-dihydroxy almond acid(3), gallic acid cetyl ester(4), protocatechuic acid(5) and ethyl gallate(7) and six flavonoids, i.e., fustin(6), 3,4′,7-trihydroxy-flavanonol(9), fisetin(13), sulfuretin(14), butin(15) and 3,7-dihydroxy flavanone-4′-rhamnose(16).关键词
漆树/木粉/抗氧化/活性成分/液质联用Key words
Rhus verniciflua Stokes/wood powder/antioxidant/active component/HPLC-MS分类
化学化工引用本文复制引用
陈虹霞,王成章,周昊,陶冉,叶建中..漆树木粉抗氧化活性成分的HPLC-MS分析[J].林产化学与工业,2017,37(1):94-100,7.基金项目
国家林业局948技术引进项目(2016-4-07) （2016-4-07）
国家国际科技合作专项(2014DFR31300) （2014DFR31300）
