临床口腔医学杂志2017，Vol.33Issue(2)：67-71,5.DOI:10.3969/j.issn.1003-1634.2017.02.002
BMP-7对大鼠骨髓间充质干细胞膜片成骨分化的影响
Effect of BMP-7 on osteogenic differentiation of rat bone marrow mesenchymal stem cells sheet
刘静 1孙洁 1何秀叶 2刘东华 3郭希民 3郭红延1
作者信息
- 1. 北京武警总医院口腔医学中心 北京 100039
- 2. 中国人民解放军总医院第一附属医院烧伤科 北京 100048
- 3. 军事医学科学院基础医学研究所 北京 100850
- 折叠
摘要
Abstract
Objective:To probe the biological properties of the contracted rat bone marrow mesenchymal stem cell ( BMMSCs) sheets and the effect of rhBMP⁃7 on osteogenic differentiation of BMMSCs sheets. Methods:Three groups were setted in the study including spontaneous differentiation group, bone induction group and BMP⁃7 suplied bone induction group. BMMSCs was harvested from SD rats and used to construct the rat BMMSCs sheets with vitamin C inducing,inverted microscope and HE staining was employed for morphological observation,CCK⁃8 assay and osteogenesis inducetion was exam⁃ined to the proliferation and differentiation of the cell sheets. Related osteogenesis gene mRNA expression was examined by RT⁃PCR. Results:After 10~14 days culture of BMMSCs in vitro,a white membranaceous sheet was formed and lifted by a⁃septic forceps. BMMSCs grew in multilayer and retained their spindle shape. HE staining showed abundant extracellular ma⁃trix(ECM). The density of cells was increased by time. Compared with BMMSCs sheet,gene expression of ALP and OCN were significantly up⁃regulated in the cell sheets induced by rhBMP⁃7. Conclusion:The osteogenesis ability of rat BMMSCs sheet induced by rhBMP⁃7 is favorable,more advantages in the bone tissue engineering.关键词
骨形成蛋白-7/骨髓间充质干细胞/细胞膜片/骨组织工程Key words
Bone morphogenetic protein 7(BMP-7)/Bone marrow mesenchymal stem cell/Cell sheet/Bone tissue engineering分类
医药卫生引用本文复制引用
刘静,孙洁,何秀叶,刘东华,郭希民,郭红延..BMP-7对大鼠骨髓间充质干细胞膜片成骨分化的影响[J].临床口腔医学杂志,2017,33(2):67-71,5.
