临床口腔医学杂志2017，Vol.33Issue(3)：131-133,3.DOI:10.3969/j.issn.1003-1634.2017.03.002
低温冻存对牙髓干细胞生物学特性的影响
The role of cryopreservation on characteristics of dental pulp stem cells
摘要
Abstract
Objective:To analyze the role of cryopreservation on self-renewal and differentiation of dental pulp stem cells(DPSCs).Methods:DPSCs from pulp tissues were isolated and cultured .Flow cytomety,cell proliferation assay and multi-lineage differentiation assay were used to compare the self-renewal and differentiation of DPSCs with or without cryopr-eservation for 1 year .Results:There was no significant difference on stem cell surface markers expression ,proliferation ,differ-entiation between DPSCs with and without cryopreservation .Conclusion:Cryopreservation failed to affect the characteristics of DPSCs,which may be one alternative strategy for long-term stem cells storage.关键词
牙髓干细胞/自我更新/多向分化/低温冻存Key words
Dental pulp stem cells/Self-renewal/Multi-lineage differentiation/Cryopreservation分类
生物科学引用本文复制引用
杨瑞莉,余婷婷,周彦恒..低温冻存对牙髓干细胞生物学特性的影响[J].临床口腔医学杂志,2017,33(3):131-133,3.基金项目
国家自然科学基金 (81600865,81470717)国家国际科技合作专项资助(2015DFB40040) （81600865,81470717）
