老年医学与保健2016，Vol.22Issue(6)：341-344,4.DOI:10.3969/j.issn.1008-8296.2016.06.05
淫羊藿苷延缓衰老过程中对ATM-Chk2-p53通路的调节作用
The regulating effect of Icariin on ATM-Chk2-p53 pathway in delaying aging
摘要
Abstract
Objective To investigate the regulating effect of icariin on ATM-Chk2-p53 pathway in old mice.Methods c57BL/6 mice of 21 months old were randomly divided into old control group and icariin treated group,and mice of 3 months old were used as young control group;mice in icariin treated group were fed with diet containing 0.02 ％ icariin for 3 months while mice in young and old control groups were fed with the standard mice diet;After 3-month treatment,a half of mice in each group were treated with 5Gy IR for 6 hours and then killed;the mRNA expression of target genes ATM,Chk2,p53 in the spleen were detected by real-time PCR;the protein levels of p-ATM,p-Chk2 and p-p53 in the spleen tissues were measured by western bloting before and after IR.Results The relative induction folds of genes such as ATM,Chk2,and p53 by IR in the spleen of old mice were significantly lower than those in young mice and the protein levels of p-ATM,p-Chk2,p-p53 in spleen tissues after IR in old mice were also much lower than those in young mice;however,icariin could up-regulate the mRNA levels of ATM,Chk2,and p53 genes and protein levels of p-ATM,p-Chk2,p-p53 in old mice treated with IR.Conclusions Icariin could activate the DNA damage checkpoint ATM-Chk2-p53 pathway,which might be an important mechanism of icariin in delaying aging.关键词
衰老/淫羊藿苷/ATM-Chk2-p53通路Key words
Aging/Icariin/ATM-Chk2-p53 pathway分类
医药卫生引用本文复制引用
陈晓亮,张素琴..淫羊藿苷延缓衰老过程中对ATM-Chk2-p53通路的调节作用[J].老年医学与保健,2016,22(6):341-344,4.基金项目
国家自然科学基金(81102681) （81102681）
