农业环境科学学报2017，Vol.36Issue(3)：466-473,8.DOI:10.11654/jaes.2016-1095
常用农药对赤子爱胜蚓急性毒性和抗氧化酶系的影响
Influence of commonly used pesticides on acute toxicity to earthworm Eisenia fetida and alteration of antioxi-dant enzyme activities
摘要
Abstract
In the present study, the acute toxicities of 11 commonly used pesticide technicals(imidacloprid, thiacloprid, avermectin, beta-cypermethrin, pymtrozine, buprofezin, chlorpyrifos, carbofuran glyphosate, atrazine and paraquat)and other 9 related pesticide formulations on Eisenia fetida were determined by using artificial soil test method. In addition, the several anti-oxidant enzymes(GST, CAT, and T-SOD) activities and MDA contents in Eisenia fetida were also determined after 14-d exposure to imidacloprid, thiacloprid and avermectin to evalu-ate the oxidative stress induced by pesticides in earthworm. The results show that the acute toxicity order of test pesticides technicals to Eise-nia fetida was:imidacloprid>thiacloprid>carbofuran>chlorpyrifos>atrazine>avermectin>glyphosate>pymtrozine>buprofezin>beta-cyperme-thrin and paraquat. According to the Guidelines of Environmental Safety Evaluation for Chemical Pesticides, imidacloprid, thiacloprid and carbofuran were classified as medium toxic grade to Eisenia fetida under laboratory conditions by using artificial soil test method, while the other 8 pesticides were classified as low toxic grade to Eisenia fetida. The results of the acute toxicities of the pesticide formulations to Eise-nia fetida showed that most of the formulations increased the toxicity to Eisenia fetida as compared to their corresponding active ingredients. Besides, the results of physiological and biochemical tests show that the CAT activity in Eisenia fetida was significantly inhibited by expo-sure to thiacloprid at 0.1 mg a.i.·kg-1 soil, while the T-SOD activity in Eisenia fetida was significantly induced by exposure to thiacloprid at the same dose level. The GST activity was significantly induced in Eisenia fetida by exposure to avermectin at 0.25 mg a.i.·kg-1 soil. The MDA content in Eisenia fetida was significantly changed by exposure to avermectin, imidacloprid and thiacloprid at 0.5, 0.1, and 0.5 mg a.i. ·kg-1, respectively. These results imply that the frequent application of pesticides might have adverse effects on the soil organisms and therefore may disturb the normal ecological function of soil.关键词
农药/土壤/蚯蚓/急性毒性/抗氧化系统Key words
pesticides/soil/earthworm/acute toxicity/antioxidant system分类
资源环境引用本文复制引用
姜锦林,单正军,周军英,卜元卿,田丰..常用农药对赤子爱胜蚓急性毒性和抗氧化酶系的影响[J].农业环境科学学报,2017,36(3):466-473,8.基金项目
"十二五"科技支撑计划(2011BAE06B09) （2011BAE06B09）
环保公益性行业科研专项(201309026) "Twelfth Five-Year"National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2011BAE06B09) （201309026）
Special Fund for Environmental Protection Scientific Research on Public Causes(201309026) （201309026）
