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石灰与黑麦草对Cu污染土壤的修复及对微生物群落的影响

杜志敏 陈国参 郭雪白 王继雯 岳丹丹 周静 巩涛 杨文玲 甄静 慕琦

农业环境科学学报2017,Vol.36Issue(3):515-521,7.
农业环境科学学报2017,Vol.36Issue(3):515-521,7.DOI:10.11654/jaes.2016-1297

石灰与黑麦草对Cu污染土壤的修复及对微生物群落的影响

Remediation of Cu contaminated soil and microbial community as affected by combining lime with ryegrass

杜志敏 1陈国参 2郭雪白 1王继雯 2岳丹丹 3周静 1巩涛 2杨文玲 1甄静 2慕琦4

作者信息

  • 1. 河南省科学院生物研究所有限责任公司,郑州 450008
  • 2. 河南省微生物工程重点实验室,郑州 450008
  • 3. 郑州工商学院,郑州450000
  • 4. 中国科学院南京土壤研究所,南京 210008
  • 折叠

摘要

Abstract

A pot experiment was set up to test the remediation effects in a Cu contaminated soil by applying of lime(SH1, SH2 and SH3, dosages of 0.1%, 0.2%and 0.4%, respectively)and ryegrass(Secale cereale), according to the determination of growth and Cu accumula-tion of ryegrass, transformation of Cu speciation in soil, and soil microbial community. The results showed that lime significantly increased biomass and Cu accumulation of ryegrass and soil pH, but decreased Cu concentration of aboveground and root of ryegrass. Compared to control, SH1, SH2 and SH3 decreased exchangeable Cu by 58.47%, 87.51% and 74.54%, while increased carbonate bound Cu to 1.36, 1.93 and 1.56 times than control, respectively. The Biolog analysis at 72 h showed that lime had the ability to increase AWCD(average well color development), S(substrate richness), Shannon's, Simpson, and McIntosh diversity index in the descending order of SH2, SH3, SH1 and CK. Lime changed the carbon source utilization patterns of soil microbial communities obviously. SH2 had the highest level of the car-bon source utilization. In conclusion, combining lime of dosage of 0.2%with ryegrass could remediate the Cu contaminated soil.

关键词

石灰/黑麦草/修复/Cu/污染/Biolog

Key words

lime/ryegrass/remediation/copper/contaminate/Biolog

分类

资源环境

引用本文复制引用

杜志敏,陈国参,郭雪白,王继雯,岳丹丹,周静,巩涛,杨文玲,甄静,慕琦..石灰与黑麦草对Cu污染土壤的修复及对微生物群落的影响[J].农业环境科学学报,2017,36(3):515-521,7.

基金项目

2017年河南省科学院基本科研业务费专项(铜尾矿耐性菌株的筛选及其对黑麦草生长影响研究) (铜尾矿耐性菌株的筛选及其对黑麦草生长影响研究)

2016年河南省科学院基本科研业务费专项(石灰对铜污染土壤微生物群落的影响研究) (石灰对铜污染土壤微生物群落的影响研究)

2012年河南省重大科技专项(121100110100) (121100110100)

2014年河南省转制机构研究发展专项[豫财政(2014)383号]The Fundamental Research Funds for Henan Academy of Sciences Institute in 2017 (2014)

The Fundamental Research Funds for Henan Academy of Sciences Institute in 2016 ()

Major science and technology projects in Henan Province in 2012(121100110100) (121100110100)

Research and development projects of Henan special transformation of institutions in 2014(383) (383)

农业环境科学学报

OA北大核心CSCDCSTPCD

1672-2043

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