农药学学报2017，Vol.19Issue(1)：18-24,7.DOI:10.16801/j.issn.1008-7303.2017.0001
粘虫抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制
Cross-resistance and biochemical resistance mechanisms of emamectin benzoate resistant population of Mythimna separate
摘要
Abstract
For effectively control of Mythimna separate Walker by reasonable application of emamectin benzoate, the cross-resistance of emamectin benzoate resistant population to other five insecticides and its biochemical resistance mechanisms were studied by bioassay and biochemical assay. The results revealed that medium level of cross-resistance to abamectin (resistance ratio: RR50 = 21.80), chlopyrifos (RR50 = 17.68) and methomyl (RR50 = 10.85), and low level of cross-resistance to phoxim (RR50 =6.00) and cyfluthrin (RR50 = 5.65) for emamectin benzoate resistant population. The enzyme inhibitors piperonyl butoxide (PBO), diethyl maleate (DEM),S,S,S-tributyl phosphorotrithioate (DEF) and triphenyl phosphate (TPP) had significant synergism to emamectin benzoate in both resistant and susceptible populations. The contents of cytochrome P450 and b5, and activities ofO-demethylase,glutathioneS-transferases and carboxylesterase of the emamectin benzoate resistant population were 3.23, 3.65, 3.63, 1.64 and 2.66-fold of that in the susceptible population, respectively. These results indicated that the increased detoxification enzyme activities may play an important role in the resistance of M. separate to emamectin benzoate.关键词
粘虫/甲氨基阿维菌素苯甲酸盐/交互抗性/生化抗性机制Key words
Mythimna separate/emamectin benzoate/cross-resistance/biochemical resistance mechanisms分类
农业科技引用本文复制引用
宋月芹,王海涛,陈玉国,王圣印,孙会忠..粘虫抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制[J].农药学学报,2017,19(1):18-24,7.基金项目
河南科技大学博士启动资金 (4026-13480047). （4026-13480047）
