农药学学报2017，Vol.19Issue(1)：32-36,5.DOI:10.16801/j.issn.1008-7303.2017.0005
埃勒斯致病杆菌SN22发酵液中Xenorhabic acid的分离
Separation of Xenorhabic acid from fermented broth of Xenorhabdus ehlersii SN22
摘要
Abstract
16SrDNA gene sequence similarity and phylogentic analysis indicated that the strain of antimicrobial activity obtained from soil belonged to the genus Xenorhabdus ehlersii and named Xenorhabdus ehlersii SN22. Xenorhabic acid (compound1) was isolated from fermented broth ofX. ehlersii SN22 by silica gel column chromatography and Sephadex LH-20 chromatography. Characterized by X-ray crystal diffraction,1HNMR and13C NMR, and ESI-MS spectral analysis, the chemical structure of1 was confirmed to be 3-(cyclohex-2-en) acrylic acid which was a new natural product. The antifungal activity of Xenorhabic acid against phytopathogenic fungi was determined by mycelium growth rate method. The results showed Xenorhabic acid could effectively inhibit the mycelium growth of Sclerotinia sclerotiorum and Pythium aphanidermatum, and the EC50 values were 27.99 and 29.55 μg/mL respectively. In addition, Xenorhabic acid also showed antibacterial activity against Escherichia coli, with the minimum inhibitory concentration (MIC) value of 62.5 μg/mL.关键词
埃勒斯致病杆菌/次生代谢产物/抑菌作用/XenorhabicacidKey words
Xenorhabdus/secondary metabolites/antimicrobial activity/Xenorhabic acid分类
农业科技引用本文复制引用
陈东升,汪钰,于志国..埃勒斯致病杆菌SN22发酵液中Xenorhabic acid的分离[J].农药学学报,2017,19(1):32-36,5.基金项目
沈阳农业大学引进人才启动基金. （）
