农药学学报2017，Vol.19Issue(1)：37-45,9.DOI:10.16801/j.issn.1008-7303.2017.0006
化学发光酶联免疫分析法同时检测3种有机磷农药残留
Development and evaluation of chemiluminescence enzyme-linked immunoassay for residue detection of three organophosphorus pesticides
摘要
Abstract
In this study, chemiluminescence enzyme-linked immunoassay (CLEIA) for the detection of the three organophosphorus pesticides was established and optimized. A monoclonal antibody with broad-specificity to parathion, parathion-methyl, and fenitrothion was employed. The direct and indirect competitive CLEIAs (dc-CLEIA and ic-CLEIA) were compared, and the physical and chemical parameters in those two reaction models were optimized. The half-maximum inhibition concentration (IC50) and the linear range from ic-CLEIA was respectively 5.57 μg/kg and 0.39-100 μg/kg for parathion, 2.30 μg/kg and 0.39-25 μg/kg for parathion-methyl, and 2.62 μg/kg and 0.39 to 25 μg/kg for fenitrothion. The half-maximum inhibition concentration (IC50) and the linear range for dc-CLEIA was respectively 5.43 μg/kg and 0.39-100 μg/kg for parathion, 1.34 μg/kg and 0.10-25 μg/kg for parathion-methyl, and 1.24 μg/kg and 0.10-25 μg/kg for fenitrothion. The novel methods for the detection of the above mentioned organophosphorus pesticides can meet the requirements of their maximum residue limits in fruits and vegetables, which paves the way to produce rapid-test kits for the detection of organophosphorus pesticides.关键词
化学发光酶联免疫分析/有机磷农药/残留/单克隆抗体/宽谱特异性Key words
chemiluminescence enzyme-linked immunoassay/organophosphorus pesticides/residue/monoclonal antibody/broad-specific分类
农业科技引用本文复制引用
邹茹冰,柳颖,王双节,张亚,郭逸蓉,朱国念..化学发光酶联免疫分析法同时检测3种有机磷农药残留[J].农药学学报,2017,19(1):37-45,9.基金项目
公益性行业 (农业)科研专项 (201203094-3). （农业）
