日用化学工业2017，Vol.47Issue(2)：90-95,108,7.DOI:10.13218/j.cnki.csdc.2017.02.007
皮肤致敏测试动物替代方法DPRA的建立和应用
Establishment and application of non-animal test method for skin sensitization (DPRA)
王滢 1吴越1
作者信息
- 1. 伽蓝(集团)股份有限公司研发中心,上海200233
- 折叠
摘要
Abstract
The identification of the skin sensitization potential is an important component of the safety assessment of cosmetic ingredients. A DPRA (Direct Peptide Reaction Assay)test method according to the specifications of ECVAM (European Centre for the Validation of Alternative Methods)was established through detection of 16 kinds of standard cosmetic ingredient. Results showed that the established DPRA test method meets the specifications of ECVAM and its sensitivity,specificity and accuracy achieves 81. 82%,100%, 77. 8% respectively. Meanwhile,for the laboratory reproducibility,the accuracy for S / NS and 4 Class classifications achieves 100% and 94. 7% respectively. Now,the method has been applied in practice and several kinds of material used for formulating cosmetic products have been detected showing skin sensitization potential.关键词
化妆品原料/皮肤致敏/动物替代方法/直接肽链反应分析Key words
cosmetic ingredient/skin sensitization/non-animal test method/Direct Peptide Reaction Assay分类
化学化工引用本文复制引用
王滢,吴越..皮肤致敏测试动物替代方法DPRA的建立和应用[J].日用化学工业,2017,47(2):90-95,108,7.
