水产学报2017，Vol.41Issue(2)：161-170,10.DOI:10.11964/jfc.20160610440
环境胁迫对大菱鲆C-型凝集素功能的影响
Effect of environmental stress on the function of C-type lectin in turbot (Scophthalmus maximus)
摘要
Abstract
C-type lectin of Scophthalmus maximus (SmLec 1) plays an important role in the non-self innate immune system.We analyzed the activity characteristics of SmLec 1,and the effect of environmental stress on its expression and regulation,and explored its potential function and response in turbot innate immune system in culture environment.In this study,the molecular mass of the SmLecl with recombinant protein was found to be 19 ku as shown by SDS-PAGE,and the target protein was obtained after purification.SmLecl showed agglutination activity against Vibrio anguillarum and Edwardsiella sp.in the six kinds of selected bacteria to test SmLecl bacterial agglutination activity.On this basis,eight sugars were taken for the inhibitory agglutination studies.Among the carbohydrates tested,lactose,D-xylose,D-fructose,sucrose and D-mannose effectively inhibited the agglutination activity of SmLecl.In physical and chemical factors (pH,high temperature),the activity of SmLec 1 was inhibited at pH ＜7,T＞ 37 ℃.QPCR was performed to examine the relative expression of the SmLecl in V.anguillarum challenge,the expression level of liver was relatively increased after pathogenic stimulation,the highest at 6 h,then gradually began to decline and returned to its original level at 32 h.Expression of SmLec1 in high temperature stress was analyzed by qPCR,and results showed that the expression of liver mRNA starts to rise from 19 ℃,and showed a decreasing trend from 22 ℃ to 27 ℃,while expression of other tissues did not change significantly.The impact of salinity stress on expression was not significant.The study could provide a scientific basis for further study of antibacterial mechanism and immune mechanism of SmLec 1.关键词
大菱鲆/C-型凝集素/鳗弧菌/蛋白重组/凝集活性/环境胁迫Key words
Scophthalmus maximus/C-type lectin/Vibrio anguillarum/recombinant proteins/agglutination activity/environmental stress分类
生物科学引用本文复制引用
夏丹丹,马爱军,黄智慧,王新安,孙志宾,崔文晓,曲江波..环境胁迫对大菱鲆C-型凝集素功能的影响[J].水产学报,2017,41(2):161-170,10.基金项目
现代农业产业技术体系专项(CARS-50-G01):中国博士后科学基金(2015M572096) （CARS-50-G01）
山东省自然科学基金(ZR2014CP001) （ZR2014CP001）
青岛市博士后科学基金(2014-2016) （2014-2016）
现代农业人才支撑计划(2016-2020)Modern Agro-Industry Technology Research System (CARS-50-G01) （2016-2020）
China Postdoctoral Science Foundation (2015M572096) （2015M572096）
Shandong Provincial Natural Science Foundation (ZR2014CP001) （ZR2014CP001）
Qingdao Postdoctoral Science Foundation funded project (2014-2016) （2014-2016）
Modern Agricultural Talent Support Program (2016-2020) （2016-2020）
