水产学报2017，Vol.41Issue(2)：171-181,11.DOI:10.11964/jfc.20160410376
凡纳滨对虾ARMC8基因的克隆及表达
Cloning and expression profile analysis of ARMC8 gene from Litopenaeus vannamei
摘要
Abstract
The purpose is to study the potential role ofLitopenaeus vannamei armadillo repeat-containing protein 8 (ARMC8) in the immune response triggered by the virus and bacterial pathogens.Full-length cDNA sequence of ARMC8 gene from L.vannamei (named LvARMC8,GenBank Accession Number:KX058562) was first cloned using RACE method.The full-length cDNA sequence of LvARMC8 was 2917 bp,which contains a 50 bp 5'UTR,822 bp 3'UTR and 2046 bp open reading frame (ORF) that encoded 681 amino acid residues.SMART analysis results showed that LvARMC8 contains six armadillo repeat (ARM) domains.Multiple alignment analysis shows that LvARMC8 shared 71％ amino acid identity with Zootermopsis nevadensis,which is the highest.Phylogenetic analysis showed that LvARMC8 was clustered together of invertebrates groups and most closely related to Tribolium castaneum ARMC8,Culex quinquefasciatus ARMC8 and Papilio xuthus ARMC8.RT-PCR analysis showed that LvARMC8 was constitutively expressed in all the examined tissues with the highest expression in epithelium and lowest expression in eyestalk.Upon WSSV challenge,the expression of LvARMC8 was significantly down-regulated at 12 hpi (hours past infection) but significantly up-regulated starting at 48 hpi,reaching the peak at 72 hpi.By V.parahaemolyticus challenging,the level of LvARMC8 expression were markedly increased at all the detected time points except 24 hpi.The result suggests that LvARMC8 might take part in the innate immune response ofL.vannamei triggered by pathogens.关键词
凡纳滨对虾/ARMC8基因/基因克隆/组织表达/WSSV感染/副溶血弧菌Key words
Litopenaeus vannamei/armadillo repeat-containing protein 8/gene cloning/tissue expression/WSSV infection/Vibrio parahaemolyticus分类
生物科学引用本文复制引用
黄金凤,杨奇慧,董晓慧,迟淑艳,刘泓宇,谭北平,章双..凡纳滨对虾ARMC8基因的克隆及表达[J].水产学报,2017,41(2):171-181,11.基金项目
广东省自然科学基金-博士启动项目(2014A030310184) （2014A030310184）
广东省自然科学基金-自由申请项目(2016A030313757) （2016A030313757）
广东普通高校青年创新人才项目(2014KQNCX079) （2014KQNCX079）
广东海洋大学优秀青年骨干教师特别资助计划(HDYQ2015004)The PhD Start-up Fund of Natural Science Foundation of Guangdong Province,China (2014A030310184) （HDYQ2015004）
Free Application Fund of Natural Science Foundation of Guangdong Province,China (2016A030313757) （2016A030313757）
Youth Innovation Talent Project of Ordinary University in Guangdong Province,China (2014KQNCX079) （2014KQNCX079）
Special Support Program for Outstanding Young Teachers of Guangdong Ocean University (HDYQ2015004) （HDYQ2015004）
