水产学报2017，Vol.41Issue(2)：182-188,7.DOI:10.11964/jfc.20160610437
河北3个日本沼虾野生群体线粒体DNA D-Loop基因序列变异及种群遗传结构分析
Genetic variation analysis of three wild populations of Oriental river prawn (Macrobrachium nipponense) in Hebei Province with mtDNA D-Loop sequences
摘要
Abstract
In order to understand further the genetic resources of Macrobrachium nipponense in South Baiyangdian (SB),North Baiyangdian (NB) and Hengshui Lake (HS) of Hebei Province,we investigated the genetic structure of Oriental river prawn M.nipponense among three different wild populations from Baiyangdian and Hengshui Lake of Hebei Province using nucleotide sequences from control region (D-Loop) gene of mitochondria.The results revealed polymorphisns at 80 nucleotide sites from an approximately 935 bp fragment of this gene region sequenced,accounting for 8.56％ of all sites,of which 77 were parsimony-informative sites,the contents of A,T,G and C of D-loop were 42.65％,36.98％,9.08％ and 11.29％,respectively,and the content of A+T (79.63％) was significantly higher than that of G+C.The fixation indices (FST) analyzed by AMOVA of ARLEQUIN Version 3.11 was 0.2336,which indicated that the variation percentages between and within groups were 23.36％ and 76.64％,respectively.NJ phylogenetic tree of 23 haplotypes constructed by Kimura-2-parameter model showed haplotypes did not cluster in accordance with the group of haploid type,but characterized by different groups with individual interlaced form complex cluster.The mismatch distribution analysis and neutrality test demonstrated that M.nipponense has not experenced a population expansion recently.关键词
日本沼虾/D-Loop基因/遗传多样性/遗传分化Key words
Macrobrachium nipponense/D-Loop sequence/wild population/genetic variation分类
生物科学引用本文复制引用
董新培,武小斌,万海付,穆淑梅,康现江,肖国华,薛建民..河北3个日本沼虾野生群体线粒体DNA D-Loop基因序列变异及种群遗传结构分析[J].水产学报,2017,41(2):182-188,7.基金项目
河北省现代农业产业技术体系淡水养殖创新团队项目 （）
Freshwater Aquaculture Innovation Team of Hebei Province Modern Agricultural Industry Technology System （）
