水产学报2017，Vol.41Issue(2)：189-197,9.DOI:10.11964/jfc.20160410373
栉孔扇贝几丁质酶基因的克隆及功能鉴定
Cloning and mineralization-related functions of the chitinase gene in Chlamys farreri
摘要
Abstract
Chitin is the main component of mollusk shell organic scaffold and its metabolism is important for shell mineralization.Chitinase with the function of chitin hydrolysis plays a key role during chitin metabolism process and is related to the formation process of shell organic scaffold.In this study,by the mass spectrum analysis of shell protein,it was proved that chitinase had an abundant amount in the shell of Chlamysfarreri.The cDNA sequence ofgene chitinase was obtained by RACE and its full length is 1587 bp,coding 439 amino acid residues.Analysis of chitinase protein sequence with bioinformatics software shows that it has a conservative function domain existing in chitinase protein family with the function of chitin hydrolysis.Chitinase in C.farreri had a much higher expression level in the mantle tissue than in other tissues,especially the area of mantle edge.In the process of shell regeneration after shell notching,the expression level of chitinase showed a decreasing tendency indicating that chitinase responded as a negative regulatory factor during shell regeneration.After knocking down the expression of chitinase,the structure of the mineral slices was irregular and fused with a fuzzy shape and unclear boundary.In conclusion,it is suggested that chitinase in C.farreri might be related to the formation of shell organic scaffold with its function of chitin hydrolysis,which is also important during the process of shell biomineralization.关键词
栉孔扇贝/几丁质酶/生物矿化/有机框架Key words
Chlamys farreri/chitinase/biomineralization/organic scaffold分类
生物科学引用本文复制引用
王俊,高静,谢军,郑向南,谢莉萍,张荣庆..栉孔扇贝几丁质酶基因的克隆及功能鉴定[J].水产学报,2017,41(2):189-197,9.基金项目
资助项目:国家“八六三”高技术研究发展计划(2012AA092204) （2012AA092204）
国家“九七三”重点基础研究发展计划(2010CB126405) （2010CB126405）
国家基础科学人才培养基金(J1310020)National High Technology Research and Development Program of China (2012AA092204) （J1310020）
National Basic Research Program of China (2010CB126405) （2010CB126405）
National Fund for Fostering Talents of Basic Science (J1310020) （J1310020）
