水产学报2017，Vol.41Issue(2)：198-211,14.DOI:10.11964/jfc.20160410372
养殖银鲳性腺发育规律和性类固醇激素变化
The gonadal development and changes of serum steroid levels of cultured Pampus argenteus
摘要
Abstract
Tissue slicing and enzyme linked immunosorbent assay (ELISA) was conducted to help understand the law of development and the level of sex steriod of cultured Pampus argenteus,and we observed the developmental characteristics of testis and ovary of P.argenteus under breeding conditions and the year-round changes in serum neutral steroids.The relation between gonadosomatic index GSI,hepatosomatic index HSI,condition factor CF and gonad development were also studied.The results showed that breeding females matured at the age of 1 year,and the matured ovary presented in the shape of "＞".Nearly half of individuals entered into stage Ⅲ in November,33.3％ individuals had been developed into stage Ⅳ.From December to next January during the winter,about 2/3 individuals degenerated to stage Ⅱ and developed quickly to stage Ⅳ in March.In April,P.argenteus reached stage Ⅴ and spawned.After spawning,the ovary entered into stage Ⅳ.The structure of testis is lobule lumen,and lobular cavities appeared in stage Ⅲ of testis,and sperm appeared in stage Ⅳ.The development of testis was faster than that of ovary,which means "male premature".20％ of individuals reached sexual maturity after 7 months.During the winter,from December to the next February with decreasing temperature,the testis degenerated to stage Ⅲ and in March the testis developed again,sperms were discharged at different time.GSI of females differed from 0.19％ to 12.89％,HSI was from 0.97％ to 2.30％ and CF ranged from 2.30％ to 3.08％.GSI in males ranged from 0.08％ to 2.62％,the value of HSI was from 0.73％ to 1.83％ and CF was from 2.11％ to 2.80％.The value of GSI reached the maximum in the stage Ⅴ,and HSI and CF reached the peak in stage Ⅳ.The content of E2 of female varied from 23.27 to 59.13 pg/mL and the content of E2 of male was 15.90 to 36.20 pg/mL in different develpomental stages and the value of HSI in females showed significant positive correlation.The content of testosterone (T) in female was from 14.57 to 68.67 nmol/L and that in male was 18.62 to 66.49 nmol/L in different develpomental stages and changes in expression levels of T showed significant positive correlation.Our research shows that the content of serum steroid hormones was closely related to the development of the testis and ovary and could serve as an important indicator to understand the development of cultured P.argenteus gonad in time.关键词
银鲳/性腺发育/性类固醇激素Key words
Pampus argenteus/gonadal development/sex steroids分类
生物科学引用本文复制引用
王腾飞,张鼎元,郭春阳,王亚军,徐善良..养殖银鲳性腺发育规律和性类固醇激素变化[J].水产学报,2017,41(2):198-211,14.基金项目
浙江省自然科学基金(LY15C190003) （LY15C190003）
宁波市重大科技专项(2015C110003)Zhejiang Provincial Natural Science Foundation (LY15C190003) （2015C110003）
Special Funds for Key Project of Science and Technology of Ningbo (2015C110003) （2015C110003）
